TNF-alpha/TNFSF2 蛋白, Human
TNF-alpha/TNFSF2 蛋白, Human是一種促炎性細胞因子,同時能夠高效地靶作用于腫瘤細胞,而對正常細胞毒性很低。
種屬:Human
表達系統(tǒng):E. coli
蛋白編號:P01375 (V77-L233)
基因 ID:7124
中文名:重組人腫瘤壞死因子-α/TNFSF2
同用名:rHuTNF-α/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factor
氨基酸序列:
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
分子量:Approximately 17.3 kDa
生物活性:The ED50 is <30 pg/mL as measured by L-929 mouse fibrosarcoma cells, corresponding to a specific activity of >3.3 × 107 units/mg.
性狀:Lyophilized powder.
組分:Lyophilized after extensive dialysis against PBS.
內(nèi)du素含量:<0.2 EU/μg, determined by LAL method.
復溶方法:It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.
保存條件 & 期限:Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
運輸條件:Room temperature in continental US; may vary elsewhere.
描述:Tumor necrosis factor α (TNF-α, also known as cachectin) is a strong pro-inflammatory cytokine playing an important role in the immune system during inflammation, cell proliferation, differentiation and apoptosis. TNF-α is type II transmembrane protein that can be expressed in both membrane-bound and secreted forms. Receptors for TNF-a are part of TNF receptor (TNFR) superfamily, with characteristic intracellular segment, a transmembrane domain, and an extracellular ligand-binding domain. They are type I transmembrane glycoproteins characterized by a cysteinerich motif (up to 40 aminoacids) repeated 2-6 times in the extracellular N-terminal domain[1]. Tumor necrosis factor-α (TNF-α) is a pleiotropic factor exhibiting antitumor effects, alters endothelial barrier function, reduces tumor interstitial pressure and mediates immune responses. TNF-α is associated with a number of cell signalling events, associated with cell necrosis and apoptosis, and is important for resistance to infection and cancer[2]
參考文獻
[1]. Zelová H, et al. TNF-α signalling and inflammation: interactions between old acquaintances. Inflamm Res. 2013 Jul;62(7):641-51.
[2]. Ren H, et al. Matrix metalloproteinase-mediation of tumor targeting human recombinant tumor necrosis factor-α fusion protein. Mol Med Rep. 2015 Aug;12(2):2035-42.
注:產(chǎn)品僅用于科研