FGF basic/bFGF 蛋白, Human (154a.a)

FGF-2/bFGF 是成纖維細(xì)胞因子家族的成員，參與骨的愈合、軟骨修復(fù)、骨修復(fù)和神經(jīng)再生。FGF-2 也是一種有絲分裂促進(jìn)劑，能加速細(xì)胞增殖。FGF-2 通過(guò)特異性靶向酪an酸激酶受體，ji活 FGF/FGFR 信號(hào)通路來(lái)調(diào)控免疫過(guò)程。例如，F(xiàn)GF-2 參與 JAK-STAT 信號(hào)通路來(lái)調(diào)控軟骨代謝，也ji活 ERK 信號(hào)促進(jìn)軟骨再生。FGF-2 結(jié)合 FGFR1/3 分別促進(jìn)關(guān)節(jié)軟骨的退化和修復(fù)。FGF-2 還是 GBM 中已知的致癌因子，有助于膠質(zhì)瘤生長(zhǎng)、血管化。重組人堿性 FGF/bFGF 蛋白 (154a.a)，是由 E.coli 大腸桿菌產(chǎn)生的重組蛋白，全長(zhǎng) 154 個(gè)氨基酸，不帶標(biāo)簽。

描述

FGF-2 is a member of the fibroblast family involved in bone healing, cartilage repair, bone repair, and nerve regeneration. FGF-2 is also a mitotic promoter that accelerates cell proliferation. FGF-2 regulates immune processes by specifically targeting tyrosine kinase receptors and activating the FGF/FGFR signaling pathway. For example, FGF-2 is involved in the JAK-STAT signaling pathway to regulate cartilage metabolism and also activates ERK signaling to promote cartilage regeneration. FGF-2 combined with FGFR1/3 promoted degeneration and repair of articular cartilage, respectively^[1]. FGF-2 is also a known carcinogen in GBM, which contributes to glioma growth and vascularization^[2].FGF basic/bFGF Protein, Human (154a.a), consists of 154 amino acids, produced by E.coli with tag free.

研究背景

FGF-2/bFGF is a member of the fibroblast family and has a high affinity for heparin. FGF-2 plays an important role in tendon to bone healing, cartilage repair, bone repair, and nerve regeneration. FGF-2 specifically binds to tyrosine kinase receptors and activates the FGF/FGFR signaling pathway. Subsequently, FGF-2 influences cell proliferation, differentiation and apoptosis, as well as immune regulation by transducing other classical pathways. For example, FGF-2 regulates the JAK-STAT signaling pathway to regulate cartilage metabolism. FGF-2 also acts as a mitotic promoter to accelerate cell proliferation. Therefore, (1) FGF-2 is an important growth factor in the healing process of ligament/tendon injury. In vitro experiments, low-dose FGF-2 can stimulate the proliferation and differentiation of bone marrow mesenchymal stem cells, and up-regulate the mRNA expression of type I/III collagen and fibronectin. However, high doses of FGF-2 did not stimulate extracellular matrix (ECM) protein proliferation and gene expression. (2) FGF-2 is also an endogenous and intrinsic growth factor in cartilage repair. FGF-2 binds to heparan sulfate proteoglycan and is stored in the ECM of articular cartilage. When cartilage is damaged or degenerated, ECM rapidly releases FGF-2 and activates ERK signaling pathways to promote cartilage regeneration. FGF-2 exhibits a biphasic effect in combination with its specific receptor. FGF-2 combined with FGFR3 promoted the repair of articular cartilage. FGF-2 combined with FGFR1 promoted the degeneration of articular cartilage^[1]. FGF-2 is expressed in granulosa cells and colliculus cells, as well as hepatocellular cancer cells, but not in non-cancerous liver tissues. This reveals the role of FGF-2 in brain tumors, particularly glioblastoma. According to studies, FGF-2 is a known carcinogenic factor in GBM. FGF-2 increases the self-renewal of glioblastoma stem cells and contributes to the growth and vascularization of glioma^[2]. FGF-2 protein is highly conserved in some species, and the similarity rate of human FGF-2 protein sequence to rat, mouse, and bovine was 97.4%, 95.45%, and 98.71%, respectively.

體外研究(In Vitro)

FGF-2 (重組人源蛋白; 3 ng/mL, 30 ng/mL; 7-28 d) 在 3 ng/mL 時(shí)觸發(fā)雙相骨髓基質(zhì)細(xì)胞 (BMSC) 反應(yīng)，在第 7 天促進(jìn)細(xì)胞增殖達(dá)到峰值，在第 14 和 28 天，增強(qiáng) I 型膠原、III 型膠原、纖維連接蛋白和 α-平滑肌肌動(dòng)蛋白 mRNA 表達(dá)。但在 30 ng/mL 時(shí)無(wú)明顯作用^[3]。

生物活性

The ED₅₀ is <0.25 ng/mL as measured by 3T3 cells, corresponding to a specific activity of >4 × 10⁶ units/mg.

種屬：Human

表達(dá)系統(tǒng)：E. coli

標(biāo)簽：Tag Free

蛋白編號(hào)：P09038-4 (A135-S288)

基因 ID：2247  [NCBI]

中文名：重組人堿性 FGF/bFGF 蛋白 (154a.a)

同用名：rHubFGF, 154a.a.; bFGF; FGF-2; HBGF-2; FGFB

氨基酸序列

AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS

分子量：Approximately 17.1 kDa

純度：Greater than 95% as determined by reducing SDS-PAGE.

性狀：Lyophilized powder

組分：Lyophilized after extensive dialysis against PBS.

復(fù)溶方法：It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

保存條件 & 期限：Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

運(yùn)輸條件：Room temperature in continental US; may vary elsewhere.

參考文獻(xiàn)

[1]. Zhang J, et al. FGF2: a key regulator augmenting tendon-to-bone healing and cartilage repair. Regen Med. 2020 Sep;15(9):2129-2142. [Content Brief]

[2]. Westermann R, et al. Basic fibroblast growth factor (bFGF), a multifunctional growth factor for neuroectodermal cells. J Cell Sci Suppl. 1990;13:97-117.  [Content Brief]

[3]. Jimenez-Pascual A, et al. FGF2: a novel druggable target for glioblastoma? Expert Opin Ther Targets. 2020 Apr;24(4):311-318.  [Content Brief]

[4]. Rusnati M, et al. Interaction of angiogenic basic fibroblast growth factor with endothelial cell heparan sulfate proteoglycans. Biological implications in neovascularization. Int J Clin Lab Res. 1996;26(1):15-23.  [Content Brief]

[5]. Hankemeier S, et al. Modulation of proliferation and differentiation of human bone marrow stromal cells by fibroblast growth factor 2: potential implications for tissue engineering of tendons and ligaments. Tissue Eng. 2005 Jan-Feb;11(1-2):41-9.  [Content Brief]