RN38-EASYspin Plus 植物RNA快速提取試劑盒
注:本產(chǎn)品僅用于科研,不可用于臨床
貨號# | 規(guī)格 | 價格 |
RN3802 | 50 次 | 910 元 |
產(chǎn)品介紹
本公司推出EASYspin無苯fen、氯仿RNA快速提取技術基礎上,又成功研發(fā)基因組DNA清除柱技術可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。裂解液迅速裂解細胞和滅活細胞RNA酶,植物RNA助提劑PLANTaid幫助結(jié)合多糖多酚并通過離心去除,然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清除柱,基因組DNA清除柱子同時吸附清除殘留的DNA, 然后RNA被選擇性洗脫濾過。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜, 再通過一系列快速的漂洗-離心的步驟, 去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, 最后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。
產(chǎn)品特點:
1.不使用有毒的苯fen,氯仿等試劑,也不需要乙醇沉淀等步驟。
2.簡捷,單個樣品操作一般可在25分鐘內(nèi)完成,簡單快速的試劑盒。
3.植物RNA助提劑可以有效結(jié)合多糖多酚,提高清除效果。
4.成功研發(fā)基因組DNA清除柱技術可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。
5.適應性極其廣泛,可以提取包括棉花、松針、冬青樹葉、葡萄葉片、等100多種國內(nèi)外試劑盒提取失敗的樣品。詳細樣品列表請參考公司主頁產(chǎn)品介紹。
6.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.2,基本無DNA殘留,可用于RT-PCR,Northern-blot和各種實驗。
使用RN38-EASYspin Plus 植物RNA快速提取試劑盒 發(fā)表的部分文獻?。。?/span>
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注:本產(chǎn)品僅用于科研,不可用于臨床