DSP Crosslinker,CAS:57757-57-0,雙端活性酯中間雙硫鍵交聯(lián)劑
DSP crosslinker (DTSP; Dithiobis[succinimidyl propionate]; Lomant's Reagent) is a homobifunctional and cleavable crosslinker that is cell membrane permeable. DSP crosslinker has amine-reactive N-hydroxysuccinimide (NHS) esters at both ends of a cleavable, 8-atom (12.0 angstrom) spacer arm, and DSP cross links occur via these NHS esters. DSP crosslinking reagent contains a reducible disulfide (thiol) bond in the spacer arm that make it a good choice for various applications. The disulfide bond may be reduced with TCEP-HCl or DTT to reverse the crosslinked proteins.
A deuterated analog of DSP is available for mass spec studies. Use &uot;light&uot; DSP-d0non-deuterated protein crosslinking reagent in bination with the &uot;heavy&uot;, deuterated analog (DSP-d8) for mass spec experiments.
DSP crosslinker must be dissolved in an organic solvent, such as DMSO or DMF, then added to an aueous crosslinking reaction. DSP is soluble in DMF, DMSO, acetone, and chloroform (up to 50mg/ml). DSP crosslinking reagent can be used to crosslink intracellular proteins and is often used for crosslinking in immunoprecipitation (IP), Co-IP and ChIP assays. If extracellular crosslinking is desired, then DTSSP crosslinker, the water soluble analog of DSP can be used.
Some precipitation of DSP crosslinking reagent can occur when diluting DMSO-dissolved DSP crosslinker in PBS. In order to minimize DSP precipitation when DMSO-dissolved DSP stock is added to PBS, gentle vortexing the PBS solution while dropwise adding the DMSO stock solution may help. Gentle warming of the reaction solution may also help minimize precipitation. If a precipitate is observed, then the protein and DSP crosslinker concentrations should be decreased. Alternatively, DMSO concentration may be increased up to 20% of the final concentration in the reaction solution.
Molecular Weight: 404.42
Spacer Length: 12.0 Å
CAS Number:57757-57-0
Alternative names: 3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester); Di(N-succinimidyl) 3,3′-dithiodipropionate; Dithiobis-succinimidyl propionate; Dithio-bis-succinimidyl propionate; Dithiobis(succinimidyl propionate); Lomant's Reagent; DSP cross-linker; DTSP crosslinker; DSP-d0
structure:
DSP Crosslinker,CAS:57757-57-0,雙端活性酯中間雙硫鍵交聯(lián)劑
西安凱新生物科技有限公司是國內(nèi)ling命科學(xué)和生物技術(shù)領(lǐng)域試劑供應(yīng)商,從成立初期就致力于為生命科學(xué)和生物技術(shù)領(lǐng)域提供優(yōu)質(zhì)產(chǎn)品和技術(shù)服務(wù),力求將的產(chǎn)品和服務(wù)奉獻給我們的客戶。目前主要產(chǎn)品有:合成磷脂、血清、高分子聚乙二醇衍生物、嵌段共聚物、磁性納米顆粒、納米金及納米金棒、近紅外熒光染料、活性熒光染料、熒光標(biāo)記的葡聚糖BSA和鏈霉親和素、蛋白交聯(lián)劑、小分子PEG衍生物、點擊化學(xué)產(chǎn)品、樹枝狀聚合物、環(huán)糊精衍生物、熱門腫瘤原料藥、大環(huán)配體類、熒光量子點、透明質(zhì)酸衍生物、石墨烯或氧化石墨烯、碳納米管、富勒烯等等,可以滿足從毫克級到公斤級的定制需求。我們非常樂于傾聽您的需求,并一如既往地繼續(xù)為您提供優(yōu)質(zhì)的產(chǎn)品和服務(wù)。
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供應(yīng)美國雙功能交聯(lián)劑(SMCC,Sulfo-SANPAH,PDPH,LC-SPDP,ANB-NOS)
我公司提供美國Proteochem交聯(lián)劑產(chǎn)品,其中包括SMCC,LC-SPDP,BS3,Sulfo-EGS,NHS-Biotin,Biotin-HPDP,Sulfo-NHS-Acetate,NHS-SS-Biotin等等產(chǎn)品試劑。
產(chǎn)品列表:
SMCC,CAS:64987-85-5
Sulfo-SMCC,CAS:92921-24-9
Sulfo-SANPAH,CAS:102568-43-4
SMPH
PDPH
Sulfo-MBS
LC-SPDP
Sulfo-LC-SPDP
MBS,CAS:526-38-3
SPDP,CAS:68181-17-9
SIA,CAS:39028-27-8
SBA,CAS:42014-51-7
SMPB,CAS:798-55-8
BMPS,CAS:55750-62-4
EMCS,CAS:55750-63-5
GMBS,CAS:80307-12-6
ANB-NOS,CAS:60117-35-3
BS3,CAS:82436-77-9
DSS,CAS:68528-80-3
DSG,CAS:79642-50-5
DSP,CAS:57757-57-0
DTSSP,CAS:81069-02-5
Sulfo-EGS,CAS:167410-92-6
EGS,CAS:70539-42-3
DST,CAS:62069-75-4
Sulfo-NHS,CAS:106627-54-7
Sulfo-NHS-Acetate,CAS:152305-87-8
Sulfo-NHS-Biotin,CAS:119616-38-5
NHS-Biotin,CAS:35013-72-0
Sulfo-NHS-LC-Biotin,CAS:127062-22-0
NHS-LC-Biotin,CAS:72040-63-2
Sulfo-NHS-SS-Biotin,CAS:325143-98-4
NHS-SS-Biotin,CAS:142439-92-7
Biotin Hydrazide,CAS:66640--6
Biotin-HPDP,CAS:129179-83-5
Iodoacetyl-LC-Biotin,CAS:93285-75-7
如果有需要Proteochem公司的產(chǎn)品,請。
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