運用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時對某個信號通路或疾病相關(guān)基因的多個基因的表達(dá)量變化進(jìn)行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號 | 名稱 | 基因列表 | 價格 |
啟因生物(wcgene.com) | HUMAN-WC025 | Signal Transduction qpcr array | DUSP6, PDE3B, PDE7A, RASSF1, FRZB, HMGCL, HMGCR, MAPK1, MAPK8, PDE5A, SLC12A1,ADRA1A, ADRA1B, ADRA1D, ADRB1, ADRB2, ADRB3, AGTR1, NPR2, NPR3, AR, EPOR, NR3C1, NR3C2. | 200元/樣 |
啟因生物(wcgene.com) | HUMAN-WC113 | Signal Transduction qpcr array | ADIPOQ, AXL, CLIC4, CSK, DOCK1, DOCK2, FYN, LYN, MAPK14, MERTK, , PIP5K1A,PRKCE, PROS1, PTEN, RAC1, RAC2, RALA, RALB, RAPGEF3, RHOA, SYK, VAV1. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC024 | Signal Transduction qpcr array | Dusp6, Pde3b, Pde7a, Rassf1, Frzb, Hmgcl, Hmgcr, Map2k5, Mapk1, Mapk8, Pde5a, Slc12a1, Adra1a, Adra1b, Adra1d, Adrb1, Adrb2, Adrb3, Agtr1a, Npr2, Npr3, Ar, Epor, Nr3c1, Nr3c2. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC025 | Signal Transduction qpcr array | Dusp6, Pde3b, Pde7a, Rassf1, Frzb, Hmgcl, Hmgcr, Mapk1, Mapk8, Pde5a, Slc12a1, Adra1a,Adra1b, Adra1d, Adrb1, Adrb2, Adrb3, Agtr1a, Npr2, Npr3, Ar, Epor, Nr3c1, Nr3c2. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC114 | Signal Transduction qpcr array | Adipoq, Axl, Clic4, Csk, Dock1, Dock2, Fyn, Lyn, Mapk14, Mertk, , Pik3cb, Pip5k1a,Prkce, Pros1, Pten, Rac1, Rac2, Rala, Ralb, Rapgef3, Rhoa, Syk, Vav1. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC112 | Signal Transduction qpcr array | Adipoq, Axl, Clic4, Csk, Dock1, Dock2, Fyn, Lyn, Mapk14, Mertk, , Pik3cb, Pip5k1a,Prkce, Pros1, Pten, Rac1, Rac2, Rala, Ralb, Rapgef3, Rhoa, Syk, Vav1. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提??;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實時定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計算PCR芯片中的各個Ct值,采用ΔΔCt方法比較基因的表達(dá)變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進(jìn)行比較。