運用高通量熒光定量PCR芯片方法,在一張96孔或384孔板上同時對某個信號通路或疾病相關(guān)基因的多個基因的表達量變化進行檢測,芯片上的基因包括了與研究對象有確定關(guān)系的基因或者待考證的基因;目前啟因生物針對不同的信號通路和疾病相關(guān)基因設(shè)計了上百種多款功能分類芯片,涵蓋生命科學(xué)研究的各個領(lǐng)域。
Real-time PCR arrays are tools that allow convenient testing of samples in many assays concurrently, parallel testing of many samples or testing of multiple samples and targets simultaneously.
——Saunders N A(2013)
品牌 | 貨號 | 名稱 | 基因列表 | 價格 |
啟因生物(wcgene.com) | HUMAN-WC062 | Insulin Signaling Pathway qpcr array | AKT1, FOXA2 (HNF3B), FOXO1, GSK3B, IGF1, IGFBP1, INSR, IRS1, MAPK1 (ERK2), MAPK8 (JNK1), MTOR,PIK3CA (p110A), PIK3R1 (PI3K p85α), PKLR, PPARGC1A (PGC-1α), PRKAA1, PTPN1, SLC2A4 (GLUT4), SOCS3, SREBF1. | 300元/樣 |
啟因生物(wcgene.com) | HUMAN-WC085 | Insulin Signaling Pathway qpcr array | AKT3, IGF1, IGF1R, IKBKB, INSR, IRS1, IRS2, MAP2K1 (MEK1), MAPK3 (ERK1), MAPK9 (JNK2), MTOR, PDE3B,PIK3CA (p110A), PIK3R1 (PI3K p85α), PPARGC1A (PGC-1α), PTPN1, RPS6KB1, SLC2A4 (GLUT4), SOCS3, SREBF1. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC061 | Insulin Signaling Pathway qpcr array | Akt1, Foxa2 (Hnf3b), Gsk3b, Igf1, Igfbp1, Insr, Irs1, Mapk1 (Erk2), Mapk8 (Jnk1), Mtor, Pik3ca (p110a),Pik3r1 (Pi3k p85α), Pklr, Ppargc1a (Pgc-1α), Prkaa1, Ptpn1, Slc2a4 (Glut4), Socs3, Srebf1. | 300元/樣 |
啟因生物(wcgene.com) | MOUSE-WC084 | Insulin Signaling Pathway qpcr array | Akt3, Gys1, Igf1, Igf1r, Ikbkb, Insr, Irs1, Irs2, Map2k1 (Mek1), Mapk3 (Erk1), Mapk9 (Jnk2), Mtor, Pde3b,Pik3ca (p110a), Pik3r1 (Pi3k p85α), Ppargc1a (Pgc-1α), Ptpn1, Rps6kb1, Slc2a4 (Glut4), Socs3, Srebf1. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC064 | Insulin Signaling Pathway qpcr array | Akt1, Foxa2 (Hnf3b), Gsk3b, Igf1, Igfbp1, Insr, Irs1, Mapk1 (Erk2), Mapk8 (Jnk1), Mtor, Pik3ca (p110a),Pik3r1 (Pi3k p85α), Pklr, Ppargc1a (Pgc-1α), Prkaa1, Ptpn1, Slc2a4 (Glut4), Socs3, Srebf1. | 300元/樣 |
啟因生物(wcgene.com) | RAT-WC086 | Insulin Signaling Pathway qpcr array | Akt3, Gys1, Igf1, Igf1r, Ikbkb, Insr, Irs1, Irs2, Map2k1 (Mek1), Mapk3 (Erk1), Mapk9 (Jnk2), Mtor, Pde3b,Pik3ca (p110a), Pik3r1 (Pi3k p85α), Ppargc1a (Pgc-1α), Ptpn1, Rps6kb1, Slc2a4 (Glut4), Socs3, Srebf1. | 300元/樣 |
檢測流程: 您只需提供樣品,其他工作交給我們。
1. 樣品RNA提?。?nbsp;
2. RNA質(zhì)量檢測,檢測RNA純度及完整度,提供RNA質(zhì)量報告;
3. cDNA合成,使用逆轉(zhuǎn)錄酶,將樣品RNA逆轉(zhuǎn)錄為cDNA;
4. 實時定量PCR反應(yīng);
5. 數(shù)據(jù)分析,計算PCR芯片中的各個Ct值,采用ΔΔCt方法比較基因的表達變化,并利用公司自主開發(fā)的數(shù)據(jù)分析軟件進行比較。