凝膠原蛋白(gelson)檢測試劑盒齊一生物科技有限公司新品*,齊一生物科技有限公司長期經營大鼠ELISA試劑盒,人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國:歡迎選購!如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
凝膠原蛋白(gelson)檢測試劑盒標本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存 過程 中 如有沉淀形成,應再次離心。
2.血漿:應根據(jù)標本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。胸腹水、腦脊液參照此實行。
4.細胞培養(yǎng)上清:檢測分泌性的成份時,用無菌管收集。離心20分鐘左右(2000-3000轉/ 分)。仔細收集上清。 檢測細胞內的成份時,用PBS(PH7.2-7.4)稀釋細胞懸液,細胞濃度達到100萬/ml左右。通過反復凍融,以 使細胞破壞并 放出細胞內成份。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。保存過程中如有沉淀形成,應再次離心。
5.組織標本:切割標本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。標本融化后仍然保?-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉/分)。仔細收集上清。分裝后一份待檢測,其余冷凍備用。
標本要求:
1.標本采集后盡早進行提取,提取按相關文獻進行,提取后應盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應避免反復凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
PAX5 (D19F8) XP? Rabbit mAb (Alexa Fluor? 488 Conjugate) 11849S 美國Cell Signaling Technology(CST)
p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor? 594 Conjugate) 11850S 美國Cell Signaling Technology(CST)
PAX5 (D19F8) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) 11852S 美國Cell Signaling Technology(CST)
PAX5 (D19F8) XP? Rabbit mAb (Alexa Fluor? 555 Conjugate) 11855S 美國Cell Signaling Technology(CST)
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (Pacific Blue?Conjugate) 11857S 美國Cell Signaling Technology(CST)
PhosphoPlus? EGFR (Tyr1068) Antibody Duet 11862S 美國Cell Signaling Technology(CST)
PSMA2 (D3A4) Rabbit mAb 11864S 美國Cell Signaling Technology(CST)
Phospho-Bad (Ser112) (40A9) Rabbit mAb (PE Conjugate) 11865S 美國Cell Signaling Technology(CST)
SignalSilence? APC2 siRNA I 11866S 美國Cell Signaling Technology(CST)
NBC1/SLC4A4 Antibody 11867S 美國Cell Signaling Technology(CST)
Phospho-GIT2 (Tyr392) (D8N9A) Rabbit mAb 11873S 美國Cell Signaling Technology(CST)
PathScan? Phospho-Bcl-2 (Ser70) Sandwich ELISA Kit 11874S 美國Cell Signaling Technology(CST)
Upf2 (D3B10) Rabbit mAb 11875S 美國Cell Signaling Technology(CST)
GABARAP Antibody 11876S 美國Cell Signaling Technology(CST)
UBE2S (D5H9H) Rabbit mAb 11878S 美國Cell Signaling Technology(CST)
SignalSilence? MDR1/ABCB1 siRNA I 11879S 美國Cell Signaling Technology(CST)
Phospho-HS1 (Tyr397) (D12C1) XP? Rabbit mAb (PE Conjugate) 11880S 美國Cell Signaling Technology(CST)
Cdc45 (D7G6) Rabbit mAb 11881S 美國Cell Signaling Technology(CST)
Ki-67 (D3B5) Rabbit mAb (Alexa Fluor? 488 Conjugate) 11882S 美國Cell Signaling Technology(CST)
Resazurin Cell Viability Kit 11884S 美國Cell Signaling Technology(CST)
Acetyl-Histone H3 (Lys36) Antibody 11885S 美國Cell Signaling Technology(CST)
Toll-like Receptor 8 (D3Z6J) Rabbit mAb 11886S 美國Cell Signaling Technology(CST)
PhosphoPlus? β-Catenin (Ser675) Antibody Duet 11887S 美國Cell Signaling Technology(CST)
TGF-β Receptor II (D3A1) Rabbit mAb 11888S 美國Cell Signaling Technology(CST)
PI3 Kinase p55 (D2B3) Rabbit mAb 11889S 美國Cell Signaling Technology(CST)
PINCH (5G7) Mouse mAb 11890S 美國Cell Signaling Technology(CST)
CHD8 (D3C1) Rabbit mAb 11891S 美國Cell Signaling Technology(CST)
Phospho-HSP27 (Ser82) (D1H2) XP? Rabbit mAb (Alexa Fluor? 647 Conjugate) 11892S 美國Cell Signaling Technology(CST)
DARPP-32 (19A3) Rabbit mAb (Alexa Fluor? 488 Conjugate) 11893S 美國Cell Signaling Technology(CST)
UCHL1 (D8R2I) XP? Rabbit mAb 11896P 美國Cell Signaling Technology(CST)
UCHL1 (D8R2I) XP? Rabbit mAb 11896S 美國Cell Signaling Technology(CST)
Phospho-NDRG1 (Ser330) (D3A12) Rabbit mAb 11899S 美國Cell Signaling Technology(CST)
PhosphoPlus? HSP27 (Ser82) Antibody Duet 11900S 美國Cell Signaling Technology(CST)
WIP1 (D4F7) Rabbit mAb 11901S 美國Cell Signaling Technology(CST)
PSMB5 Antibody 11903S 美國Cell Signaling Technology(CST)
IRF-3 (D6I4C) XP? Rabbit mAb 11904P 美國Cell Signaling Technology(CST)
IRF-3 (D6I4C) XP? Rabbit mAb 11904S 美國Cell Signaling Technology(CST)
SignalSilence? MDR1/ABCB1 siRNA II 11906S 美國Cell Signaling Technology(CST)
PAI-1 (D9C4) Rabbit mAb 11907S 美國Cell Signaling Technology(CST)
MDH2 (D8Q5S) Rabbit mAb 11908S 美國Cell Signaling Technology(CST)
Phospho-cdc25C (Ser216) Blocking Peptide 1190S 美國Cell Signaling Technology(CST)
EWS Antibody 11910S 美國Cell Signaling Technology(CST)
NFI-C Antibody 11911S 美國Cell Signaling Technology(CST)
CHD4 (D8B12) Rabbit mAb 11912S 美國Cell Signaling Technology(CST)
PC1/3 Antibody 11914S 美國Cell Signaling Technology(CST)
ENSA (D5Z1U) Rabbit mAb 11915S 美國Cell Signaling Technology(CST)
Chelerythrine Chloride 11916S 美國Cell Signaling Technology(CST)
Phospho-Rb (Ser807/811) (D20B12) XP? Rabbit mAb (PE Conjugate) 11917S 美國Cell Signaling Technology(CST)
Phospho-c-Fos (Ser32) (D82C12) XP? Rabbit mAb (PE Conjugate) 11919S 美國Cell Signaling Technology(CST)
DYRK2 (D9A3K) Rabbit mAb 11921S 美國Cell Signaling Technology(CST)
RBX1 (D3J5I) Rabbit mAb 11922S 美國Cell Signaling Technology(CST)
Mitofusin-2 (D1E9) Rabbit mAb 11925S 美國Cell Signaling Technology(CST)
PTK7 Antibody 11926S 美國Cell Signaling Technology(CST)
Phospho-IRAK4 (Thr345/Ser346) (D6D7) Rabbit mAb 11927S 美國Cell Signaling Technology(CST)
SENP1 (D16D7) Rabbit mAb 11929S 美國Cell Signaling Technology(CST)
IKKα (3G12) Mouse mAb 11930S 美國Cell Signaling Technology(CST)
USP14 (D8Q6S) Rabbit mAb 11931S 美國Cell Signaling Technology(CST)
Image-iT? FX Signal Enhancer 11932S 美國Cell Signaling Technology(CST)
CAD Antibody 11933S 美國Cell Signaling Technology(CST)
"四氫呋喃
Tetrahydrofuran" 99.5%,Water< 0.005% (by K.F.),
with molecular sieves, stabilized with BHT
"N,N-二甲基甲酰胺
N,N-Dimethylformamide" 99.8%,Water< 0.005% (by K.F.),
with molecular sieves
"二甲亞砜
Dimethyl sulfoxide" 99.7%,Water< 0.005% (by K.F.),
"1,4-二噁烷
1,4-Dioxane" 99.5%,Water< 0.005% (by K.F.),
with molecular sieves, stabilized with BHT
"吡啶
Pyridine" 99.5%,Water< 0.005% (by K.F.),
with molecular sieves
"1-甲基-2-吡咯烷酮
1-Methyl-2-pyrrolidinone" "99.5%, Water<0.005% (by K.F.),
with molecular sieves"
氘代氯仿, Chloroform-d 99.8 atom%D, stabilized with silver foil
氘代氯仿, Chloroform-d 99.8atom%D, contains 0.03% v/v TMS
氘代氯仿, Chloroform-d 99.8atom%D
氘代氯仿 99.8atom%D,contains 0.03% v/v TMS
氘代二甲亞砜,Dimethyl sulfoxide-d6 99.8 atom%D, contains 0.03% TMS
重水, Deuterium oxide 99.8 atom % D
重水, Deuterium oxide 99.9atom%D
氘代甲醇, Methanol-d4 99.8 atom % D
氘代丙酮, Acetone-d6 99.8atom%D
氘代吡啶, Pyridine-d5 99 atom% D
氘代乙酸, Acetic acid-d 98atom% D
"氘代四氫呋喃
Tetrahydrofuran-d8" 99.5 atom% D
"氘代苯
Benzene-d6" 99.5 atom%D
"氘代氫化鋁鋰
Lithium aluminum deuteride" 97% , [98 atom% D]
"氘代硼氫化鈉
Sodium borodeuteride" 99 atom%D
Acetonitrile" 99.9%,Water< 0.001% (by K.F.)
"四氫呋喃
Tetrahydrofuran" 99.9%,Water< 0.005% (by K.F.),
stabilized with BHT
"N,N-二甲基甲酰胺
N,N-Dimethylformamide" 99.8%,Water< 0.005% (by K.F.)
"二甲亞砜
Dimethyl sulfoxide" 99.7%,Water< 0.005% (by K.F.)
"1,4-二噁烷
1,4-Dioxane" 99.8%,Water< 0.005% (by K.F.),
stabilized with BHT
"乙酸乙酯
Ethyl acetate" Water< 0.005% (by K.F.)
Acetonitrile" 99.9%,Water< 0.005% (by K.F.),
with molecular sieves
"四氫呋喃
Tetrahydrofuran" 99.5%,Water< 0.005% (by K.F.),
with molecular sieves, stabilized with BHT
"N,N-二甲基甲酰胺
N,N-Dimethylformamide" 99.8%,Water< 0.005% (by K.F.),
with molecular sieves