細(xì)胞絨毛蛋白埃茲蛋白(CVL)檢測試劑盒(血清.血漿.組織)齊一生物科技(上海)有限公司專業(yè)經(jīng)營ELISA試劑盒產(chǎn)品種類齊全、質(zhì)量可靠、*、靈敏度高、效果穩(wěn)定、易保存、操作簡單.到貨快、確保每一個(gè)ELISA試劑盒質(zhì)量.凡是購買我公司ELISA試劑盒免費(fèi)代測、提供技術(shù)服務(wù)、5個(gè)工作日出實(shí)驗(yàn)結(jié)果.歡迎新老客戶前來: !
細(xì)胞絨毛蛋白埃茲蛋白(CVL)檢測試劑盒(血清.血漿.組織)標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆?。?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測,其余冷凍備用。
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
Anti-5-HTR3(5-hydroxytryptamine (serotonin) receptor 3a)
Anti-5-HTR4(5 hydroxytryptamine serotonin receptor 4)
Anti-5-LOX (5-lipoxygenase)
Anti-8-OHdG(8-Hydroxy-2'-deoxyguanosine; 8-Hydroxydeoxyguanosine)
Anti-A2AR/Adenosine A2A-R(A2A adenosine receptor)
Anti-AACT-α1 (chymotrypsin-Alpha1)
Anti-AAK1(AP2 associated protein kinase 1)
Anti-AARS2(alanyl tRNA synthetase 2)
Anti-AARS2(alanyl tRNA synthetase 2)
Anti-AAT(Alpha-1 Antitiypsin)
Anti-AATK(Apoptosis associated tyrosine kinase)
Anti-AATF(Apoptosis-antagonizing transcription factor)
Anti-ABCA1(ATP binding cassette transporter A1)
Anti-ABCB5/(ATP-binding cassette sub-family B member 5)
Anti-ABCB6(ATP-binding cassette sub-family B member 6)
Anti-ABCF1(ATP-binding cassette sub-family F (GCN20) member 1)
Anti-ABCG1(ATP-binding cassette sub-family G member 1)
Anti-ABCG1(ATP-binding cassette sub-family G member 1)
Anti-ABCG2/BCRP/BXP-21(breast cancer resistance protein)
Anti-ABCG4(ATP binding cassette, subfamily G member 4)
Anti-ABL1(v-abl Abelson murine leukemia viral oncogene homolog 1)
Anti-ABL2/ARG(v-abl Abelson murine leukemia viral oncogene homolog 2)
Anti-ABI1/SSH3BP1(Abelson interactor 1)
Anti-ABP(Androgen Binding Protein)
Anti-ACE1( ACE, testis-specific isoform precursor)
Anti-ACE2(Angiotensin converting enzyme 2)
Anti-ACEI (Angiotensin I Converting Enzyme Inhibitor )
Anti-ACD/PTOP(Adrenocortical dysplasia protein)
Anti-Acinus (Apoptotic chromatin condensation Inducer in the nucleus)
Anti-Ack1
Anti-ACTH (Adrenorticotrophin hormons 1-39)
Anti-ACTH (Adrenorticotrophin hormons 18-39)
Anti-ACTH (Adrenorticotrophin hormons 7-23)
Anti-Actin α /α-SMA (Actin alpha , smooth muscle aorta)
Anti-α-ACTN4(α-Actinin 4)
Anti-F-Actin(filament actin)
Anti-AD7C-NTP/NTP/AF (Neurual thread protein)
Anti-AD7C-NTP/NTP/AF (Neurual thread protein)human
Anti-AD7C-NTP/NTP/AF(Neurual thread protein)
Anti-ADAM-TS1/METH1(a disintegrin and metalloproteinase with-thrombospodin type 1)
Anti-ADAM-TS7 (A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 7)
Anti-ADAM-TS7 (A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 7)
Anti-ADAM-TS12(A Disintegrin-like and Metalloprotease with Thrombospondin Type 1 Motif, 12)
Anti-Adducin protein
Anti-ADFP/ADRP/adipophilin (adipose differentiation-related protein)
Anti-AEV(Avian Encephalomyelitis virus)
Anti-ADH/AVP(Antidiuretic Hormone;Arginine Vasopressin)
Anti-Adipo R1 (adiponectin receptor 1)
Anti-Adipo R2 (adiponectin receptor 2)
Anti-Adiponectin
Anti-ADM/AM (Adrenomedullin)
Anti-ADM (Adrenomedullin)ProAM-N20
Anti-ADM R (Adrenomedullin receptor)
Anti-ADNP/NAP (Activity-dependent Neuroprotective protein )
Anti-ADORA3/A3AR(adenosine receptor A3)
Anti-ADRA2 (alpha 2 adrenergic receptor)
Anti-AHR(aryl hydrocarbon receptor)
Anti-ADRB1 (β1-adrenergic receptor)
Anti-ADRB2 (β2-adrenergic receptor )
Anti-ADRB3/β3-AR (β3-adrenergic receptor)
Anti-AE3/SLC4A3(anion exchanger protein 3)
Anti-AEBP1 (Adipocyte enhance binding protein 1)
Cleaved-SUMO-2/3 (G93) Polyclonal Antibody YC0075 美國Immunoway
Cleaved-TACE (R215) Polyclonal Antibody YC0076 美國Immunoway
Cleaved-Thrombin APII (R327) Polyclonal Antibody YC0077 美國Immunoway
Cleaved-Tumstatin (P1426) Polyclonal Antibody YC0078 美國Immunoway
Ephrin-A5 Polyclonal Antibody YC0079 美國Immunoway
Cleaved-Factor B Bb (K260) Polyclonal Antibody YC0080 美國Immunoway
Cleaved-Factor VII LC (R212) Polyclonal Antibody YC0081 美國Immunoway
Cleaved-Factor X/ Factor X LC (A41) Polyclonal Antibody YC0082 美國Immunoway
Cleaved-Factor Xa activated HC (I235) Polyclonal Antibody YC0083 美國Immunoway
Cleaved-Factor XII HC (I20) Polyclonal Antibody YC0084 美國Immunoway
Cleaved-Factor XII HC (R372) Polyclonal Antibody YC0085 美國Immunoway
Cleaved-GGT4 HC (T472) Polyclonal Antibody YC0086 美國Immunoway
HABP2 Polyclonal Antibody YC0087 美國Immunoway
Cleaved-Integrin α5 HC (F42) Polyclonal Antibody YC0088 美國Immunoway
Cleaved-Integrin α5 LC (E895) Polyclonal Antibody YC0089 美國Immunoway
Cleaved-Integrin α6 LC (E942) Polyclonal Antibody YC0090 美國Immunoway
Cleaved-Integrin α7 LC (E959) Polyclonal Antibody YC0091 美國Immunoway
Cleaved-Integrin αV HC (K889) Polyclonal Antibody YC0092 美國Immunoway
Cleaved-ITI-H1 (D672) Polyclonal Antibody YC0093 美國Immunoway
Cleaved-ITI-H2 (D702) Polyclonal Antibody YC0094 美國Immunoway
Cleaved-ITI-H4 70k (R661) Polyclonal Antibody YC0095 美國Immunoway
Cleaved-Kininogen-1 HC (K380) Polyclonal Antibody YC0096 美國Immunoway
Cleaved-MASP-1 HC (R448) Polyclonal Antibody YC0097 美國Immunoway
MMP-1 Polyclonal Antibody YC0098 美國Immunoway
Cleaved-MMP-1 22k (F100) Polyclonal Antibody YC0099 美國Immunoway
Cleaved-MPO 89k (A49) Polyclonal Antibody YC0100 美國Immunoway
Cleaved-PARP-1 (D214) Polyclonal Antibody YC0101 美國Immunoway
Cleaved-Plasma Kallikrein HC (R390) Polyclonal Antibody YC0102 美國Immunoway
Cleaved-Plasminogen HC A short form (V98) Polyclonal Antibody YC0103 美國Immunoway
Cleaved-Thrombin R (S42) Polyclonal Antibody YC0104 美國Immunoway
Caspase-6 Polyclonal Antibody YC0105 美國Immunoway
Myc-tag Polyclonal Antibody YG0001 美國Immunoway
His-tag Polyclonal Antibody YG0002 美國Immunoway
HA-tag Polyclonal Antibody YG0003 美國Immunoway
FLAG-tag Polyclonal Antibody YG0004 美國Immunoway
Acetyl-Histone H1 (K25) Polyclonal Antibody YK0001 美國Immunoway
Acetyl-Histone H2A (K5) Polyclonal Antibody YK0002 美國Immunoway
Acetyl-Histone H2B (K5) Polyclonal Antibody YK0003 美國Immunoway
Acetyl-Histone H2B (K12) Polyclonal Antibody YK0004 美國Immunoway
Acetyl-Histone H2B (K15) Polyclonal Antibody YK0005 美國Immunoway
Acetyl-Histone H3 (K9) Polyclonal Antibody YK0006 美國Immunoway
Acetyl-Histone H3 (K14) Polyclonal Antibody YK0007 美國Immunoway
Acetyl-Histone H3 (K18) Polyclonal Antibody YK0008 美國Immunoway
Acetyl-Histone H3 (K23) Polyclonal Antibody YK0009 美國Immunoway
Acetyl-Histone H3 (K27) Polyclonal Antibody YK0010 美國Immunoway
Acetyl-Histone H4 (K5) Polyclonal Antibody YK0011 美國Immunoway
Acetyl-Histone H4 (K8) Polyclonal Antibody YK0012 美國Immunoway
Acetyl-Histone H4 (K12) Polyclonal Antibody YK0013 美國Immunoway
Acetyl-Histone H4 (K16) Polyclonal Antibody YK0014 美國Immunoway
Acetyl-p53 (K319) Polyclonal Antibody YK0015 美國Immunoway
Acetyl-p53 (K381) Polyclonal Antibody YK0016 美國Immunoway
Acetyl-p53 (K386) Polyclonal Antibody YK0017 美國Immunoway
Acetyl-NFκB-p65 (K310) Polyclonal Antibody YK0018 美國Immunoway
Acetyl-CBP (K1535) Polyclonal Antibody YK0019 美國Immunoway
Acetyl-Lys proteins Polyclonal Antibody YK0020 美國Immunoway
4E-BP1 Monoclonal Antibody YM0001 美國Immunoway
4E-BP1 Monoclonal Antibody YM0002 美國Immunoway
4E-BP1 Monoclonal Antibody YM0003 美國Immunoway
A1BG Monoclonal Antibody YM0004 美國Immunoway
AAT Monoclonal Antibody YM0005 美國Immunoway
AATM Monoclonal Antibody YM0006 美國Immunoway
ABCG2 Monoclonal Antibody YM0007 美國Immunoway
ABCG2 Monoclonal Antibody YM0008 美國Immunoway
ACTA2 Monoclonal Antibody YM0009 美國Immunoway
ACTA2 Monoclonal Antibody YM0010 美國Immunoway
ACTA2 Monoclonal Antibody YM0011 美國Immunoway
Actin β Monoclonal Antibody YM0012 美國Immunoway
A-FABP Monoclonal Antibody YM0013 美國Immunoway
A-FABP Monoclonal Antibody YM0014 美國Immunoway
AFP Monoclonal Antibody YM0015 美國Immunoway
AGT Monoclonal Antibody YM0016 美國Immunoway
AIF-M1 Monoclonal Antibody YM0017 美國Immunoway