齊一生物人登革熱抗體（DF-Ab）試劑盒

齊一生物人登革熱抗體（DF-Ab）試劑盒齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,種類齊全,價(jià)格實(shí)惠,貨期短.購(gòu)買我公司產(chǎn)品新老客戶,可享全程技術(shù)指導(dǎo)：(包括售前標(biāo)本收集、后期實(shí)驗(yàn)、實(shí)驗(yàn)結(jié)束后數(shù)據(jù)分析等技術(shù)問題,我公司會(huì)有專業(yè)的技術(shù)即時(shí)給您去電溝通、解決.)

試劑盒名稱：齊一生物人登革熱抗體（DF-Ab）試劑盒
規(guī) 格：48T/96T
品牌：進(jìn)口原裝/分裝/國(guó)產(chǎn)
產(chǎn)品的用途：僅供科研究使用！
價(jià)格：電議,咨詢?cè)诰€客服,到我司qysw@qiyibio.。齊一生物科技（上海）有限公司提供大量品牌進(jìn)口原裝、以及穩(wěn)定性強(qiáng)的國(guó)產(chǎn)ELISA試劑盒，性價(jià)比*歡迎您前來(lái)選購(gòu)！
操作步驟
實(shí)驗(yàn)開始前，請(qǐng)?zhí)崆芭渲煤盟性噭?，試劑或樣品稀釋時(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔?？瞻卓准訕悠废♂屢?00μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
8.         用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)。

Anti-Ambra1
Anti-CRABP2
Anti-DMAP1
Anti-UVRAG
Anti-Dapper homolog 1
Anti-APG16L
Anti-GPR18
Anti-Transglutaminase 2 (TG2)
Anti-AROS
Anti-Sphingolipid Receptor Edg6/S1P4
Anti-DAAM1
Anti-SINTBAD/TBKBP1
Anti-APG10L
Anti-Sphingolipid Receptor Edg3/S1P3
Anti-p35/IL-12A
Anti-Relaxin receptor 1/LGR7
Anti-EBI3
Anti-Vang-like 1
Anti-NDRG1
Anti-RGS5
Anti-RGS5
Anti-Homeobox protein D13 (HOXD13)
Anti-THEM2
Anti-GNPDA1
Anti-G Protein-Coupled Receptor GPR35
Anti-FMN2
Anti-Latexin
Anti-ERAS
Anti-GNPDA1
Anti-Mesenchymal stem cell protein DSCD75
Anti-CNOT4
Anti-TYRO3
Anti-SOD
Anti-Catalase
Anti-GR (2- Glutathione reductase)
Anti-SWAP70
Anti-Mucin 13
Anti-FNBP1
Anti-FMN2
Anti-EPM2A/Laforin
Anti-Latexin
Anti-AXL/UFON
Anti-FAM59A (C18orf11)
Anti-Embigin
Anti-Syndecan-4
Anti-Snx2
Anti-eIF4A2
Anti-eIF4B
Anti-Syncollin
Anti-Phospho-TLR4/CD284 pTyr674
Anti-eIF4A2
Anti-CUEDC2
Anti-Leucine rich alpha 2 glycoprotein 1 (LRG1)
Anti-ROR alpha
Anti-ROR alpha
Anti-IkZF3, Zinc finger protein Aiolos
Anti-TLR4
Anti-Spi-B
Anti-THEM2
Anti-eIF4A1
Anti-UCP4 (uncoupling protein4)
Anti-AIRE/APECED
Anti-Als2Cr2
Anti-CUEDC2
Anti-eIF3B
Anti-Tis7
Anti-MERTK
Anti-Snx2
Anti-PRAT4A
Anti-HES1
Anti-TLR4
Anti-eIF3A
Anti-eIF4B
Anti-eIF3G
Anti-Histone H3.3
Anti-TOB2
Anti-TIP60
Anti-PACS-1
Anti-TLR3 (ligand binding domain)
Anti-eIF3D
Anti-SLAP
Anti-UNC5CL
Anti-ARRB2
Anti-EBI3
Anti-RAP2A
Anti-SUCNR1/GPR91
Anti-G6B
Anti-GARP/LRRC32
Anti-ACAP2
Anti-G6D
Anti-NALP7
Human anti-B burgdorleri antibody,BB-Ab ELISA試劑盒
Human Anti-smooth muscle antibody,ASMA ELISA試劑盒
Human anti-liver cell membrane antibody,LMA ELISA試劑盒
Human liver specific lipoprotein,LSP ELISA試劑盒
Human eye muscle antibody,EMAb ELISA試劑盒
Human Anti-Complement 1q antibody,C1q ELISA試劑盒
Human intrinsic factor antibody,IFA ELISA試劑盒
Human anti-phospholipid antibody,Apl/APA ELISA試劑盒
Human anti-Histone antibody,AHA ELISA試劑盒
Human crystal proteins α,Cryα ELISA試劑盒
Human crystal proteins β,Cryβ ELISA試劑盒 
Human Collagen autoantibody,CLA ELISA試劑盒
Human anti-filaggrin antibody,AFA ELISA試劑盒
Human anti-cyclic citrullinated peptide,CCP ELISA試劑盒
Human anti-perinuclear factor,APF ELISA試劑盒
Human Anti-nuclear Antibody,ANA ELISA試劑盒
Human liver kidney microsome autoantibody,LKM ELISA試劑盒
Human InterferonαAntibody,IFNα-Ab ELISA試劑盒
Human Sc1-70 Antibody,Sc1-70-Ab ELISA試劑盒
Human Jo-1 Antibody,Jo1/HRS ELISA試劑盒
Human anti-ribonucleo protein Antibody,RNP-Ab ELISA試劑盒
Human natural deoxyribonucleic acid antibody,n-DNA-Ab ELISA試劑盒
Human anti-extractable nuclear antigen antibody,ENA-Ab ELISA試劑盒
Human deoxyribonucleic protein antibody,DNP-Ab ELISA試劑盒
Human anti-Actin antibody,AAA ELISA試劑盒
Human heparin associated antibody,HIT ELISA試劑盒
Human Proteinase 3 Antibody,PR3Ab ELISA試劑盒
Human Proteinase 3 Antibody,PR3Ab ELISA試劑盒
Human β anti-galactan proteins IgG ELISA試劑盒
Human β anti-galactan proteins IgG ELISA試劑盒
Human anti-Endomysial Antibody IgA,EMA IgA ELISA試劑盒
Human anti-glycoprotein antibody,GP ELISA試劑盒
Human anti-Monocyte antibody,AMA ELISA試劑盒
Human oxidized lowdensity lipoprotein antibody,OLAb ELISA試劑盒 
Human anti-EJ-antibody，EJ/GlyRS ELISA試劑盒
Human LDL-IC ELISA試劑盒
Human proliferating cell nuclear antigen antibody,PCNA ELISA試劑盒
Human anti-IgE receptor antibody ELISA試劑盒
Human anti-IVIgG antibody ELISA試劑盒
Human Anti-myocardial antibody,AMA ELISA試劑盒
human Anti-Thyroid Microsome Antibody,ATMA/TMAB ELISA試劑盒
human Anti-Thyroid Microsome Antibody,ATMA/TMAB ELISA試劑盒
human glutamic acid decarboxylase autoantibody,GAD-Ab ELISA試劑盒
Human plaet antibodies IgG/M/A,PA-IgG/M/A ELISA試劑盒
Human anti-lymphocyte globulin,ALG ELISA試劑盒
Human Anti-trypsin,AT ELISA試劑盒
Human anti-ribosomal P protein antibody,ARPA/Rib-P ELISA試劑盒
Human anti-ribosomal P protein antibody,ARPA/Rib-P ELISA試劑盒
Human Anti-Somatic Muscle Antibody,ASA ELISA試劑盒
Human Thyroid Antibody,TAb ELISA試劑盒
Human islet cell antibody,ICA ELISA試劑盒 
Human salivary duct autoantibody,SDA ELISA試劑盒
Human mitotic spindle apparatus antibody,MSA ELISA試劑盒
Human Anti-keratin antibody,AKA ELISA試劑盒
Human Perinuclear anti-neutrophil cytoplasmic antibody,pANCA ELISA試劑盒
human anti-neutrophil cytoplasmic antibody,cANCA ELISA試劑盒
Human anti-prothrombins antibodies,aPT1/aPT2 ELISA試劑盒
human phosphatidylinositol antibody IgG/IgM,PI Ab-IgG/IgM ELISA試劑盒
Human β2-glycoprotein 1 antibody IgA/G/M,β2-GP1 IgA/G/M ELISA試劑盒
Human Cathepsin Antibodies,Cath Ab ELISA試劑盒
Human Lactoferrin antibody IgG,LF/LTF Ab-IgG ELISA試劑盒
Human anti-Bactericidal permeability increasing protein antibody,BPI-Ab ELISA試劑盒
Human Mouse myeloperoxidase-antineutrophil cytoplasmic antibody IgG,MPO-ANCA IgG ELISA試劑盒
Human Anti-proteinase 3 antibody IgG,PR3 Ab-IgG ELISA試劑盒
Human anti-alpha-Fodrin IgG/IgA,α-Fodrin IgG/IgA ELISA試劑盒 
human single stranded DNA Antibody,ssDNA ELISA試劑盒
Human histone H2A-H2B-DNA autoantibody ELISA試劑盒
Human anti-double stranded DNA,dsDNA ELISA試劑盒
human anti-nucleosome antibody IgG,AnuA-IgG ELISA試劑盒
Human anti-scl70 antibody,SCL70/topoⅠELISA試劑盒
Human SS-B/La Antibody,ELISA試劑盒
Human Pemphigus Foliaceus,PF ELISA試劑盒
Human β2-Glycoprotein 1 antibody IgA,β2-GP1 Ab IgA ELISA試劑盒