現(xiàn)貨產(chǎn)品人纖維連接素相關(guān)抗原(FRA)ELISA檢測(cè)試劑盒齊一生物科技有限公司新品*,齊一生物科技有限公司長(zhǎng)期經(jīng)營大鼠ELISA試劑盒,人elisa試劑盒,小鼠elisa試劑盒等種屬試劑盒,歡迎您訪問我們的,www.qiyibio。。com全國:歡迎選購!如有什么問題請(qǐng)給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議
產(chǎn)品名稱:現(xiàn)貨產(chǎn)品人纖維連接素相關(guān)抗原(FRA)ELISA檢測(cè)試劑盒
規(guī)格:96T/48T
檢測(cè)標(biāo)本:血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測(cè)方法:ELISA
檢測(cè)類型:酶聯(lián)免疫夾心法
運(yùn)輸保存:2~8°保存6個(gè)月
齊一生物主要經(jīng)營進(jìn)口原裝Elisa試劑盒全國:,種類齊全,價(jià)格實(shí)惠,購買齊一生物產(chǎn)品的新老客戶,可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),可檢測(cè)人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報(bào)價(jià),可齊一生物.
特點(diǎn)
一、高效、靈敏、特異的抗體;
二、穩(wěn)定的重復(fù)性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相載體;
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清:操作過程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿:EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液:1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿:將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存:如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.
此IBL試劑盒能用于小鼠血清,EDTA血漿,細(xì)胞上清中產(chǎn)品定量檢測(cè)試劑盒成分
1預(yù)包被板:抗小鼠白介素-6兔子IgG,親合純化96T
2酶標(biāo)記抗體:(30倍濃縮)HRP標(biāo)記抗小鼠白介素-6兔子IgG,親合純化0.4mLx1
3標(biāo)準(zhǔn)品:重組小鼠白介素-60.5mLx2
4EIA緩沖液:含1%BSA,0.05%吐溫20BPS30mLx1
5標(biāo)記抗體稀釋液:含1%BSA,0.05%吐溫20BPS12mLx1
6顯色劑:TMB底物液15mLx1
7終止液:1N硫酸12mLx1
8濃縮洗滌液:(40倍濃縮)含1%BSA,0.05%吐溫20BPS50mLx1操作說明1實(shí)驗(yàn)所需器材(但試劑盒沒有提供)酶標(biāo)儀(450nm)微移液管及其吸嘴量筒及燒杯去離子水冰箱(4°C)坐標(biāo)紙(log/log)吸水紙?jiān)嚬?用于標(biāo)準(zhǔn)品稀釋)溫育箱(37°C±1°C)洗瓶(用于洗板)一次性試劑管(用于濃縮酶標(biāo)記抗體和顯色劑)550750 MS H-2KB-IG PURE RECOM DIMERX 0.25MG
550751 MS H-2LD-IG PURE RECOM DIMERX 0.25MG
550756 TRML TRNSFRSE FITC SET 100TST
550757 TRML TRNSFRSE PE SET 100TST
550759 HU CD63 NHP FITC MAB 50TST H5C6
550760 HU CD34 PURE MAB 0.1MG 563
550761 HU CD34 PE MAB 100TST 563
550762 HAM IGG2 LAM ITCL PERCP-CY5.5 .1MG HA4/8
550763 HAM IGG1 KPA ITCL PERCP-CY5.5 .1MG A19-3
550764 RAT IGG2B ITCL PERCP-CY5.5 0.1MG A95-1
550765 RAT IGG2A ITCL PERCP-CY5.5 0.1MG R35-95
550767 RAT IG PE 0.2MG POLY1271
550768 CVTS 12X47 MM FOR MONOLIGHT 2001 1000CT
550772 MS CD94 PURE MAB 0.1MG 18D3
550773 MS CD94 BIOTIN MAB 0.1MG 18D3
550780 NUCLEOSOME PURE MAB 50UG 11E6
550781 POLY ADP-RIBOSE (PAR) PURE MAB 50UG 10H
550783 HU CYCLIN B1 PE SET 100TST
550787 HU CD14 PERCP-CY5.5 MAB 0.1MG M5E2
550795 MS IGG1 KPA PERCP-CY5.5 0.1MG MOPC-21
550797 MS CD8B PURE MAB 0.1MG H35-17.2
550798 MS CD8B PE MAB 0.1MG H35-17.2
550799 HU IL-6 OPTEIA KIT 2PLT
550802 RAT CD45.1 FITC MAB 0.1MG NDS58
550803 BRDU IN-SITU DTEC KIT 50SLIDES
550804 HU CD138 PURE MAB 0.1MG DL-101
550805 HU CD138 PE MAB 100TST DL-101
550806 HU BLD GROUP A PURE MAB 0.1MG NAM87-1F6
550807 HU BLD GROUP A FITC MAB 0.1MG NAM87-1F6
550810 HU LAIR-1 PURE MAB 0.1MG DX26
550811 HU LAIR-1 PE MAB 100TST DX26
550814 HU CD244 PURE MAB 0.1MG 2-69
550815 HU CD244 FITC MAB 100TST 2-69
550816 HU CD244 PE MAB 100TST 2-69
550821 ACT CASPASE-3 PE MAB 100TST C92-605
550825 PI/RNASE STAINING BUF 100ML
550826 MS IG APC PAB 0.1MG POLY1270
550834 HU P16 IHC MAB 1.0ML G175-405
550838 MLH-1 IHC PURE MAB 1.0ML G168-15
550840 HPV-16 L1 IHC PURE MAB 1.0ML CAMVIR-1
550843 HU ESPNL MBP IHC PURE MAB 1.0ML AHE-2
550851 HU CD11A NHP PE MAB 50TST HI111
550852 HU CD11A NHP APC MAB 50TST HI111
550853 HU HLA-DR DP DQ NHP FITC MAB 50TST TU39
550854 MS IGG1 KPA ITCL NHP APC 50TST MOPC-21
550855 HU CD45RA APC MAB 100TST HI100
550856 HU CD195 APC MAB 100TST 3A9
550859 HU A20 PURE MAB 50UG E5-1619
550863 MS KLRG1 BIOTIN MAB 0.1MG 2F1
550866 HU CD62P NHP FITC MAB 50TST AC1.2
550868 HU CD16B PE MAB 100TST CLB-GRAN11.5
550874 MS IGG1 APC MAB 0.1MG X56
550875 RBT IGG STD PURE PAB 0.1MG POLY1281
550878 MS IGG1 KPA ITCL IHC PURE 1.0ML MOPC-31C
550880 DAB SBSTR KIT
550881 MS IGM PERCP-CY5.5 MAB 0.1MG R6-60.2
550882 MS IGG2A KPA ITCL APC .1MG G155-178
550883 MS IGM KPA ITCL APC 0.1MG G155-228
550884 RAT IGG1 LAM ITCL APC MAB 0.1MG A110-1
550888 HU CD62P APC MAB 100TST AK-4
550889 HU CD206 APC MAB 100TST 19.2
550890 HU CD137 APC MAB 100TST 4B4-1
550891 BRDU BUF 25MG
550900 HU CD120A BIOTIN MAB 0.5MG MABTNFR1-B1
550906 HU CDW329 FITC MAB 100TST E10-286
550911 ANXN V-FITC FLUORESCENCE MICROSCOPY KIT
550912 HU CD253 NALE MAB 0.25MG RIK-2
550913 HU CYCLIN A PE SET 100TST
550914 ACT CASPASE-3 APOP PE KIT 100TST
550920 HU CDC25C PURE MAB 50UG TC113
550924 MS IGA KPA ITCL PURE MAB 0.5MG MOPC-320
550926 HU IP-10 OPTEIA SET 20TST
550927 MS IGG2A ITCL PERCP-CY5.5 0.1MG G155-178
550930 HU ACT CASP-3 OPTEIA SET 5TST
550931 HU IGG APC MAB 100TST G18-145
550937 HU (CD197) CCR7 PURE MAB 0.25MG 2H4
550938 RBT IGM BIOTIN MAB 0.2MG 367.2
550944 HU UBIQUITIN ASCI 100UL 6C1.17
550946 SAV-HRP PRE-DILUTED 50ML
550947 HU C4A OPTEIA KIT 1PLT
550948 HU CD253 OPTEIA SET 20TST
550950 MS IL-6 OPTEIA KIT 2PLT
550951 HU CTKN 14-15-16-19 PURE MAB 0.1MG KA4
550953 CYTOKERATIN 14,15,16,19 PE SET 100TST
550954 MS CD4 PERCP-CY5.5 MAB 0.1MG RM4-5
550955 HU CD79B APC MAB 100TST CB3-1
550956 HU CD36 APC MAB 100TST CB38
550959 JURKAT APOPTOTIC LYSATE SET I 1.0MG
550961 RAT ERYTRD CELLS PURE MAB 0.1MG HIS49
550962 RAT ERYTRD CELLS BIOTIN MAB 0.1MG HIS49
550963 MS IGM ITCL PURE MAB 0.5MG PMG1-146.1
550966 MS CD72ABD ALOATG FITC MAB 0.1MG K10.6
550967 HU CD183 APC MAB 100TST 1C6/CXCR3
550968 HU CD161 APC MAB 100TST DX12
550970 RAT CD8B BIOTIN MAB 0.1MG 341
550972 RAT CD11A PE MAB 0.1MG WT.1
550973 RAT CD28 FITC MAB 0.1MG JJ319
550974 RAT CD44H FITC MAB 0.1MG OX-49
550976 RAT CD59 FITC MAB 0.1MG TH9
550977 RAT CD134 BIOTIN MAB 0.1MG OX-40
550978 RAT CD161A BIOTIN MAB 0.1MG 10/78
550979 RAT CD6 PAN-T CELLS PURE MAB 0.1MG OX-52
550981 RAT RT1 AAB FITC MAB 0.1MG C3
550982 RAT RT1D FITC MAB 0.1MG OX-17
550986 MS LY-49F BIOTIN MAB 0.1MG HBF-719
550987 MS LY-49F PE MAB 0.1MG HBF-719
550988 HU CD44 PURE MAB 0.1MG 515
550989 HU CD44 PE MAB 100TST 515
550990 HU CD44 NALE MAB 0.5MG 515
550992 MS CD19 APC MAB 0.1MG 1D3
550993 MS CD11B PERCP-CY5.5 MAB .1MG M1/70
550994 MS CD45 PERCP-CY5.5 MAB 0.1MG 30-F11
550995 HU LT-ALP OPTEIA SET 20TST
550998 HU MIG OPTEIA SET 20TST
550999 HU IL-8 OPTEIA KIT II 2PLT