齊一生物微量轉(zhuǎn)鐵蛋白(MTF)ELISA檢測試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個試劑盒都經(jīng)過嚴格的質(zhì)檢控制,包括:板內(nèi)、板間實驗精度,靈敏度,回收率,穩(wěn)定性等指標的測定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測體系簡單而直接,可根據(jù)450 nm波長的讀值迅速計算定量結(jié)果。試劑盒中提供的檢測抗體能夠識別全長靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測結(jié)果更穩(wěn)定可靠
齊一生物微量轉(zhuǎn)鐵蛋白(MTF)ELISA檢測試劑盒標本要求:
1.標本采集后盡早進行提取,提取按相關(guān)文獻進行,提取后應(yīng)盡快進行實驗。若不能馬上進行試驗,可將標本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
注意事項:
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標包被板開封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會有結(jié)晶析出,稀釋時可在水浴中加溫助溶,洗滌時不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對其準確性,以避免試驗誤差。一次加樣時間控制在5分鐘內(nèi),如標本數(shù)量多,推薦使用排槍加樣。
4. 請每次測定的同時做標準曲線,做復(fù)孔。如標本中待測物質(zhì)含量過高(樣本OD值大于標準品孔*孔的OD值),請先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測定,計算時請zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請避光保存。
7. 嚴格按照說明書的操作進行,試驗結(jié)果判定必須以酶標儀讀數(shù)為準.
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號組分不得混用。
10. 如與英文說明書有異,以英文說明書為準。
計算:
以標準物的濃度為橫坐標,OD值為縱坐標,
在坐標紙上繪出標準曲線,根據(jù)樣品的OD
值由標準曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標準物的濃度與OD值計算出標
準曲線的直線回歸方程式,將樣品的OD值
代入方程式,計算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實際濃度。
試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見應(yīng)分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個月 齊一生物科技有限公司主要經(jīng)營進口原裝Elisa試劑盒,種類齊全,價格實惠,購買我公司產(chǎn)品的新老客戶,可享免費代測實驗服務(wù),可檢測人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本。全程提供。如有什么問題請給我們或者留言,告訴我您的需要,并留下您寶貴的意見和建議。Human Placenta Cadherin,P-cad ELISA試劑盒
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