齊一生物現(xiàn)貨紅細(xì)胞刺激因子(ESF)檢測(cè)試劑盒新近推出多種雙抗夾心法ELISA(酶聯(lián)免疫吸附法)試劑盒,具有特異性高、檢測(cè)范圍廣、靈敏度高和變異系數(shù)小等優(yōu)勢(shì),是定量檢測(cè)多種生物樣本中靶蛋白(如細(xì)胞裂解液、血漿、血清等)的理想工具
齊一生物現(xiàn)貨紅細(xì)胞刺激因子(ESF)檢測(cè)試劑盒一、高效、靈敏、特異的抗體;
二、穩(wěn)定的重復(fù)性和可靠性;
三、吸附性能好,空白值低,孔底透明度高的固相載體;
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型;
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說明方法的準(zhǔn)確度.比如水中總無機(jī)氯含量測(cè)定,樣品水中含有無機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟:
1)抗原表位的計(jì)算機(jī)篩選;
2)抗原的制備;
3)血清的采集;
4)間接ELISA方法的建立;
5)間接ELISA方法的敏感性和特異性驗(yàn)證;
6)試劑盒的穩(wěn)定性驗(yàn)證;
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.Pierce MA191859 TBP Associated Factor 1 50 μg
Pierce MA191860 alpha Actinin 100 μg
Pierce MA191861 Adenovirus type 5 E1A 250 μl
Pierce MA191863 Secretory Component Glycoprotein 500 μl
Pierce MA191867 Bone Alkaline Phosphatase 100 μg
Pierce MA191869 IKB alpha cleaved 50 μg
Pierce MA191870 KSR 50 μg
Pierce MA191878 Pierce(R) Anti-DYKDDDDK Epitope Tag Mouse Monoclonal 100 μg
Pierce MA1918781MG FLAG Epitope Tag Antibody (FG4R) Large Pack Size 1MG(1MG/ML)
Pierce MA191878BTIN FLAG Tag mAb, Biotin Conjugate 50UG(1MG/ML)
Pierce MA191878D488 FLAG D488 Conjugated Antibody 50 UG
Pierce MA191878D550 Flag D550 Conjugated Antibody 50 UG
Pierce MA191878D650 FLAG Tag mAb, DyLight 650 Conjugated, 50 ug (1 mg/ml) 50UG(1MG/ML)
Pierce MA191878D680 FLAG Tag mAb, DyLight 680 Conjugated, 50 ug (1 mg/ml) 50UG(1MG/ML)
Pierce MA191878HRP FLAG HRP Conjugated Antibody 50 UG.
Pierce MA191880 Dopamine 100 μg
Pierce MA191883 Haloperidol 250 μg
Pierce MA191884 Human IgE (AP) 250 μl
Pierce MA191895 Bestrophin 100 μl
Pierce MA191896 Caspase 7 100 μl
Pierce MA191897 NQO1 100 μl
Pierce MA1930 ATP Synthase beta 100 μg
Pierce MA1932 Recoverin 100 μg
Pierce MA1934 Calcium Sensing Receptor 100 μg
Pierce MA1935 CFTR 100 μl
Pierce MA1936 DIABLO 100 μg
Pierce MA1940 Galectin 3 100 μg
Pierce MA1950 Bcl-3 100 μg
Pierce MA1952 eGFP Epitope Tag 100 μg
Pierce MA19587 beta-3 Tubulin 50 ug
Pierce MA1980 Pierce(R) c-Myc Mouse Monoclonal Antibody, 100 ug 100 μg
Pierce MA19801MG c-Myc Antibody (9E10) Large Pack Sz. (1 mg/ml) 1EA(1MG/ML)
Pierce MA1980HRP Anti-c-myc mAb, HRP Conjugated 100 UG
Pierce MA1981 S-peptide Epitope Tag 100 μg
Pierce MA2310 Mesothelioma 25 μg
Pierce MA2607 ANTI-RAT CD26 MONOCLONAL 500 UG
Pierce MA2910 ANTI-HUMAN CD29 MONOCLONAL 200 UG
Pierce MA3002 BAP31 100 μl
Pierce MA3003 BAP31 100 μl
Pierce MA3004 VCP 100 μl
Pierce MA3005 Endothelin 1 100 μl
Pierce MA3006 HSP70/Heat Shock Protein 70 50 μl
Pierce MA3007 HSP70/Heat Shock Protein 70 250 μl
Pierce MA3008 HSP70/Heat Shock Protein 70 50 μl
Pierce MA3009 HSP70/Heat Shock Protein 70 50 μl
Pierce MA3010 HSP90/Heat Shock Protein 90 alpha 100 μl
Pierce MA3011 HSP90/Heat Shock Protein 90 alpha 400 μl
Pierce MA3012 HSP60/Heat Shock Protein 60 100 μg
Pierce MA3013 HSP60/Heat Shock Protein 60 100 μg
Pierce MA3014 HSC70/Heat Shock Cognate Protein 70 (13D3) 50 μl
Pierce MA3015 HSP27/Heat Shock Protein 27 100 μl
Pierce MA3016 GRP94/HSP90B1 100 μl
Pierce MA3018 PDI 100 μl
Pierce MA3019 PDI 100 μl
Pierce MA3020 Endothelin 100 μl
Pierce MA3021A Calcitonin (Lot 034-111) 100 μg
Pierce MA3022 HSP60/Heat Shock Protein 60 100 μl
Pierce MA3023 HSP60/Heat Shock Protein 60 100 μl
Pierce MA3024 NFATc1 100 μl
Pierce MA3026 TCP1 100 μl
Pierce MA3027 Calnexin 100 μl
Pierce MA3028 HSP70/Heat Shock Protein 70 100 μl
Pierce MA3029 Cdc37 100 μl
Pierce MA3030 uNOS 200 μl
Pierce MA3032 Cytochrome P450 3A4/3A5 100 μl
Pierce MA3033 Cytochrome P450 3A5 100 μl
Pierce MA3034 Cytochrome P450 3A7 100 μl
Pierce MA3036 Cytochrome P450 1A1/1A2 100 μl
Pierce MA3037 Cytochrome P450 1A2 100 μl
Pierce MA3041 Acetylcholinesterase 100 μl
Pierce MA3042 Acetylcholinesterase 200 μl
Pierce MA3043 Nicotinic Acetylcholine Receptor 100 μl
Pierce MA3044 Muscarinic Acetylcholine Rec. M2 250 μl
Pierce MA3060 ADP-ribosylation factor 100 μl
Pierce MA3061 alpha Adaptin 500 μl
Pierce MA3062 beta-1 Spectrin 100 μl