5-TAMRA Picolyl Azide,Alexa Fluor 546

5-TAMRA Picolyl Azide,Alexa Fluor 546
目前Cyanine系列染料以兩種異構(gòu)體的形式存在，一種是非磺化花青素（non-sulfonated cyanines），一種是磺化花青素（sulfonatedcyanine）。兩者的光譜性質(zhì)幾乎*，因此大部分應(yīng)用可相互替換。

5-TAMRA Picolyl Azide,Alexa Fluor 546

產(chǎn)品介紹

5-TAMRA Picolyl Azide

5-TAMRA Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concen*ration of Cu(I) at the reaction site. The chelating effect of picolyl azide moiety to raise the effective concen*ration of copper at the reaction site is so great that it leads to an increase of signal intensity of up to 40-fold. In addition, the use of 5-TAMRA Picolyl Azide instead of conventional 5-TAMRA Azide allows for at least a tenfold reduction in the concen*ration of the copper catalyst without sacrificing the efficiency of labeling.

Although the mixed isomers of 5(6)-TAMRA is a preferred, routinely used red fluorescent dye for staining proteins, labeling peptides and nucleotides with TAMRA mixed isomers might be troublesome due to significant signal broadening in HPLC purification. Peptides and nucleotides labeled with a single isomer TAMRA usually give better resolution in HPLC purification that is often reuired in the conjugation processes.

In summary, the in*roduction of a picolyl moiety into an azide probe leads to a substantial increase in the sensitivity of alkyne detection. This will be of special value for the detection of low abundance targets or where significant increase in signal intensity is desired.

Abs/Em Maxima	545/576
Extinction coefficient	91,000
Flow cytometry laser line	532 or 555 nm
Microscopy laser line	532 or 555 nm
Spectrally similar dyes	Alexa Fluor® 546, Atto™ 543, CF™ 555 Dye, DyLight™549
Molecular weight	808.89
CAS	N/A
Solubility	DMSO, DMF, MeOH
Purity	>97% (HPLC)
Appearance	Red solid
Storage conditions	-20C
Shipping conditions	Ambient temperature

結(jié)構(gòu)式：

 

5-TAMRA Picolyl Azide,Alexa Fluor 546

西安凱新生物科技有限公司

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炔烴 Dye alkynes

1-Ethynyl pyrene

3-Ethynyl perylene

BDP FL alkyne

FAM alkyne, 5-isomer

FAM alkyne, 6-isomer

TAMRA alkyne, 5-isomer

BDP TMR alkyne

Cyanine3 alkyne

Cyanine5 alkyne

Cyanine5.5 alkyne

Cyanine7 alkyne

Cyanine7.5 alkyne

Sulfo-Cyanine3 alkyne

Sulfo-Cyanine5 alkyne