1,25二羥基維生素D3（1,25-（OH）2D3DVDDHVD 3）檢測(cè)試劑盒齊一生物科技有限公司產(chǎn)品的新老客戶，可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù)，可檢測(cè)人源，小鼠，大鼠，豬，狗，猴，兔，羊，雞，牛，鴨子，魚，植物等各種樣本.全程提供.如需其他Elisa試劑盒說明書及報(bào)價(jià)，可齊一生物科技有限公司.全國：歡迎選購

1,25二羥基維生素D3（1,25-（OH）2D3DVDDHVD 3）檢測(cè)試劑盒操作步驟
實(shí)驗(yàn)開始前，請(qǐng)?zhí)崆芭渲煤盟性噭噭┗驑悠废♂寱r(shí)，均需混勻，混勻時(shí)盡量避免起泡。每次檢測(cè)都應(yīng)該做標(biāo)準(zhǔn)曲線。如樣品濃度過高時(shí)，用樣品稀釋液進(jìn)行稀釋，以使樣品符合試劑盒的檢測(cè)范圍。
1.         加樣：分別設(shè)空白孔、標(biāo)準(zhǔn)孔、待測(cè)樣品孔。空白孔加樣品稀釋液100μl，余孔分別加標(biāo)準(zhǔn)品或待測(cè)樣品100μl，注意不要有氣泡，加樣將樣品加于酶標(biāo)板孔底部，盡量不觸及孔壁，輕輕晃動(dòng)混勻，酶標(biāo)板加上蓋或覆膜，37℃反應(yīng)120分鐘。
為保證實(shí)驗(yàn)結(jié)果有效性，每次實(shí)驗(yàn)請(qǐng)使用新的標(biāo)準(zhǔn)品溶液。
2.         棄去液體，甩干，不用洗滌。每孔加生物素標(biāo)記抗體工作液 100μl（取1μl生物素標(biāo)記抗體加99μl生物素標(biāo)記抗體稀釋液的比例配制，輕輕混勻，在使用前一小時(shí)內(nèi)配制），37℃,60分鐘。
3.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板3次，每次浸泡1-2分鐘，350μl/每孔，甩干。
4.         每孔加辣根過氧化物酶標(biāo)記親和素工作液（同生物素標(biāo)記抗體工作液） 100μl，37℃，60分鐘。
5.         溫育60分鐘后，棄去孔內(nèi)液體，甩干，洗板5次，每次浸泡1-2分鐘，350μl/每孔，甩干。
6.         依序每孔加底物溶液90μl，37℃避光顯色（30分鐘內(nèi)，此時(shí)肉眼可見標(biāo)準(zhǔn)品的前3-4孔有明顯的梯度藍(lán)色，后3-4孔梯度不明顯，即可終止）。
7.         依序每孔加終止溶液50μl，終止反應(yīng)（此時(shí)藍(lán)色立轉(zhuǎn)黃色）。終止液的加入順序應(yīng)盡量與底物液的加入順序相同。為了保證實(shí)驗(yàn)結(jié)果的準(zhǔn)確性，底物反應(yīng)時(shí)間到后應(yīng)盡快加入終止液。
1,25二羥基維生素D3（1,25-（OH）2D3DVDDHVD 3）檢測(cè)試劑盒8.         用酶聯(lián)儀在450nm波長(zhǎng)依序測(cè)量各孔的光密度（OD值）。 在加終止液后15分鐘以內(nèi)進(jìn)行檢測(cè)

Rotor-Gene Q Software    9019101    qiagen
PyroMark Assay Design SW 2.0 (5)    9019079    qiagen
PyroMark Thermoplate, PyroQ96MD    9019071    qiagen
Tray, EZ1xl    9019017    qiagen
Reflector, UV lamp, EZ1xl    9019008    qiagen
Adapter, 24x5ml flat base Tubes    9018934    qiagen
X-Motor DC, Gear, 20V    9016014    qiagen
BioSprint 15 tube tray    9016003    qiagen
Tube Holder, STS MDx, 15 mm (12)    9015972    qiagen
Luminex Maintenance Kit    9015910    qiagen
EZ1 DNA PRFN Card    9015862    qiagen
Rings (10), pinching, 4.5/2.7 mm    9015687    qiagen
Cable, Interface, 3000 mm, PC to X-main    9015613    qiagen
Upgrade, LiquiChip IS 2.x    9015606    qiagen
Door, plete    9015536    qiagen
Lead screw - Y, PyroQ96MD    9019165    qiagen
Reagent chamber lid, PyroQ96MD    9019163    qiagen
Channeling Adapter BR8000    9013928    qiagen
QIAsoft 4.x, BR8000 Cust. Edition    9013865    qiagen
Adapter, deep-well plate, BS96    9021191    qiagen
Controller, Galil Motion, DCU    9021052    qiagen
Cap, Reagent Bottle, DCU    9021009    qiagen
Motor, Pipettor Z, DCU    9021005    qiagen
Foot assembly, SMC, QGX    9020911    qiagen
Optoswitch, X axis, C34    9020716    qiagen
HSV PCR REAGENT I    1061117    qiagen
Syringe Pump, RCS1    9016566    qiagen
Cable REDGE/Gateway, RS232, Kloehn    9016487    qiagen
Computer, standard, XP    9016306    qiagen
Hardware Upgrade Kit, Universal Tissue    9016233    qiagen
Valve - Release, PyroQ96    9019183    qiagen
Lens array, PyroQ96ID    9019138    qiagen
PyroMark Q96 MD Software    9019085    qiagen
Sample rack, EZ1xl    9019016    qiagen
Reagent Block, 24x1.5ml Tapered-Base    9018955    qiagen
Adapter, Light Cycler 480 96-Well Plate    9018937    qiagen
QIAsoft 4.1.4.8 Servicepack    9016114    qiagen
Board, YZ6L, master, BR8 AT3/B10    9016048    qiagen
Software QIAsoft MDx Service Edition    9015913    qiagen
Fittings (10), M6/2.8 mm, white    9015686    qiagen
Board, power branching    9015528    qiagen
Board, operation panel    9015527    qiagen
Vacuum Pump (100 V), PyroQ24VW    9021234    qiagen
Skin Panel, Waste Drawer, DCU    9021114    qiagen
Skin Panel, Lower Center Rear, DCU    9021100    qiagen
Vial Robot Assist Assembly, DCU    9020996    qiagen
Kit, calibration, mech. variation, RG3    9020937    qiagen
Pulley, x idler belt tensioner, C34    9020708    qiagen
Spindle nut - XY, PyroQ24    9020691    qiagen
Display, keypad, GSC    9020487    qiagen
Kit, fuse AS, Qsym    9020390    qiagen
PM kit, C12 & QIAgility, CG1    9020316    qiagen
Packaging, foam base, C1x    9020265    qiagen
Z-pulley, idler ass., 8 ch. gearbox, Cx8    9020234    qiagen
Belt, Y-axis assembly, Cx8    9020228    qiagen
Fitting,Peek,10-32,1/16tubing (5)    9239525    qiagen
Vacuum Pump (110-120 V), PyroQ24VW    9020164    qiagen
Mikrowin data reduction Software    9232820    qiagen
Cal. Set for Reader 3 000 12 00    9232807    qiagen
Manual, user, QS 4.1 (WW)    9236528    qiagen
#Agreement, Plato 3, PM, 1 yr    9236486    qiagen
Travel Charge, zone A    9236446    qiagen
1.0 hr    9236439    qiagen
Holder, tip rack 2    9011884    qiagen
Power Supply, 400W, BR8, RD v1.0    9236982    qiagen
X/Y table, PyroQ96ID    9019142    qiagen
Camera MX5, PyroQ96ID    9019128    qiagen
Heating block, PyroQ96ID    9019127    qiagen
Pin, piercing, EZ1    9019027    qiagen
Worktable, side wall, right, EZ1xl    9019026    qiagen
Master Mix Block, 5x5ml Tubes    9018959    qiagen
Reagent Block, 16x0.2ml/1.5ml PCR/FlatBa    9018952    qiagen
Adapter, COBAS TaqMan 48 Plates    9018941    qiagen
Precision Pipetting Calibration Kit    9015617    qiagen
EZ1 RNA Card    9015590    qiagen
Cover, back ,type C    9015533    qiagen
9231793    Motor, gripper, handler, BR8000    
9238690    Computer board Zent2, no Eprom    
9231434    Probe, 0,4mm, ceramic coated for PK80    
9020071    Keypad, TLyser    
9020070    Controller 100-120V/200-240V, TLyser    
9014996    Shaker, Highspeed Unit, BR8 RD    
9014870    X-Motor, BR8    
9014790    Tip Disposal Bags MDx    Autoclavable bags for collection and disposal of used disposable tips, pack of 15
9014642    System Reagent Troughs (Qty 10)    
9019188    Mirror, PyroQ96    
1064181    EGFR RGQ PCR Kit (24) IUO    
1017647    Carrier RNA(poly A) 12x1350μg-15-25°C,KG    
1059454    ResPlex II Plus Panel (25) PRE    
1055628    TopElute Fluid (60/60),KG    
1061068    Sample Preparation Reagents, 30ml    
1061104    UU PCR REAGENT I    
9016583    ZLV Pipetting Posit., RCS1    
9016561    BR MDx DSP Serv.pro Plasticware Kit, V1    
9016505    Rect. Bucket Set (4)    
9016486    Cable Dilutor bus, RS485, Kloehn    
9016288    Cable, RS232, EZ1a    
1,25二羥基維生素D3（1,25-（OH）2D3DVDDHVD 3）檢測(cè)試劑盒9019181    PCB 7 - Connection, PyroQ96MD    
9019115    Fan - IPC, PyroQ96ID    
9019101    Rotor-Gene Q Software    
9019079    PyroMark Assay Design SW 2.0 (5)    "All-in-one software for design of both PCR and sequencing primers

Five additional software licenses for PyroMark AssayDesign SW 1.0"
9019071    PyroMark Thermoplate, PyroQ96MD    Holder for placement of PyroMark Q96 Plate on heat block during annealing step prior to PyroMark Q96 MD analysis.
9019017    Tray, EZ1xl    
9019008    Reflector, UV lamp, EZ1xl    
9018934    Adapter, 24x5ml flat base Tubes    
9016014    X-Motor DC, Gear, 20V    
9016003    BioSprint 15 tube tray    "he 5-tube strips sit on this tray during the run.
see page 33 of the user manual for visuals"
9015972    Tube Holder, STS MDx, 15 mm (12)    
9015910    Luminex Maintenance Kit    
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
9015687    Rings (10), pinching, 4.5/2.7 mm    
9015613    Cable, Interface, 3000 mm, PC to X-main    
9015606    Upgrade, LiquiChip IS 2.x    
9015536    Door, plete    
9019165    Lead screw - Y, PyroQ96MD    
9019163    Reagent chamber lid, PyroQ96MD    
9013928    Channeling Adapter BR8000    
9013865    QIAsoft 4.x, BR8000 Cust. Edition    
9021191    Adapter, deep-well plate, BS96    
9021052    Controller, Galil Motion, DCU    
9021009    Cap, Reagent Bottle, DCU    
9021005    Motor, Pipettor Z, DCU    
9020911    Foot assembly, SMC, QGX    
9020716    Optoswitch, X axis, C34    
1061117    HSV PCR REAGENT I    
9016566    Syringe Pump, RCS1    
9016487    Cable REDGE/Gateway, RS232, Kloehn    
9016306    Computer, standard, XP    
9016233    Hardware Upgrade Kit, Universal Tissue    
9019183    Valve - Release, PyroQ96    
9019138    Lens array, PyroQ96ID    
9019085    PyroMark Q96 MD Software    For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.
9019016    Sample rack, EZ1xl    
9018955    Reagent Block, 24x1.5ml Tapered-Base    
9018937    Adapter, Light Cycler 480 96-Well Plate    
9016114    QIAsoft 4.1.4.8 Servicepack    
9016048    Board, YZ6L, master, BR8 AT3/B10    
9015913    Software QIAsoft MDx Service Edition    
9015686    Fittings (10), M6/2.8 mm, white    
9015528    Board, power branching    
9015527    Board, operation panel    
9021234    Vacuum Pump (100 V), PyroQ24VW    
9021114    Skin Panel, Waste Drawer, DCU    
9021100    Skin Panel, Lower Center Rear, DCU    
9020996    Vial Robot Assist Assembly, DCU    
9020937    Kit, calibration, mech. variation, RG3    
9020708    Pulley, x idler belt tensioner, C34    
9020691    Spindle nut - XY, PyroQ24    
9020487    Display, keypad, GSC    
9020390    Kit, fuse AS, Qsym    
9020316    PM kit, C12 & QIAgility, CG1    
9020265    Packaging, foam base, C1x    
9020234    Z-pulley, idler ass., 8 ch. gearbox, Cx8    
9020228    Belt, Y-axis assembly, Cx8    
9239525    Fitting,Peek,10-32,1/16tubing (5)    
9020164    Vacuum Pump (110-120 V), PyroQ24VW    
9232820    Mikrowin data reduction Software    
9232807    Cal. Set for Reader 3 000 12 00    
9236528    Manual, user, QS 4.1 (WW)    
9236486    #Agreement, Plato 3, PM, 1 yr    An agreement for preventive maintenance provides coverage for travel, labor, and parts, plus the cost of consumables required for the maintenance visit. Work carried out during the preventive 1,25二羥基維生素D3（1,25-（OH）2D3DVDDHVD 3）檢測(cè)試劑盒maintenance visit is fully documented in accordance with GMP/GLP directives.
9236446    Travel Charge, zone A    
9236439    1.0 hr    
9011884    Holder, tip rack 2    
9236982    Power Supply, 400W, BR8, RD v1.0