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- 程成
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- 021-60348496
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- 18121453965
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- 上海市松江加工園區(qū)
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- www.qiyibio.com
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鞭毛蛋白(flagellin)檢測(cè)試劑盒(血清.血漿.組織)提供免費(fèi)代測(cè)的服務(wù):(您只需把標(biāo)本寄過來,我們?yōu)槟?jié)省時(shí)間,幫您出結(jié)果,原始數(shù)據(jù),分析數(shù)據(jù)均可提供,實(shí)驗(yàn)時(shí)間五個(gè)工作日給您結(jié)果.如有什么問題請(qǐng)給我們來電021-60348496021-60348467或者留言,告訴我您的需要,并留下您寶貴的意見和建議,齊一生物全體員工將全心全意為您服務(wù)!
鞭毛蛋白(flagellin)檢測(cè)試劑盒(血清.血漿.組織)標(biāo)本的采集及保存:
1.血清:室溫血液自然凝固10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存 過程 中 如有沉淀形成,應(yīng)再次離心。
2.血漿:應(yīng)根據(jù)標(biāo)本的要求選擇EDTA、檸檬酸鈉或肝素作為抗凝劑,混合10-20分鐘后,離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
3.尿液:用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。胸腹水、腦脊液參照此實(shí)行。
4.細(xì)胞培養(yǎng)上清:檢測(cè)分泌性的成份時(shí),用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/ 分)。仔細(xì)收集上清。 檢測(cè)細(xì)胞內(nèi)的成份時(shí),用PBS(PH7.2-7.4)稀釋細(xì)胞懸液,細(xì)胞濃度達(dá)到100萬/ml左右。通過反復(fù)凍融,以 使細(xì)胞破壞并 放出細(xì)胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。保存過程中如有沉淀形成,應(yīng)再次離心。
5.組織標(biāo)本:切割標(biāo)本后,稱取重量。加入一定量的PBS,PH7.4。用液氮迅速冷凍保存?zhèn)溆谩?biāo)本融化后仍然保持2-8℃的溫度。加入一定量的PBS(PH7.4),用手工或勻漿器將標(biāo)本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細(xì)收集上清。分裝后一份待檢測(cè),其余冷凍備用。
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
雞γ干擾素(IFN-γ)ELISA試劑盒
雞免疫球蛋白A(IgA)ELISA試劑盒
雞免疫球蛋白M(IgM)ELISA試劑盒
雞分泌型免疫球蛋白A(SIgA)ELISA試劑盒
雞心肌肌鈣蛋白I(cTNI)ELISA試劑盒
雞白介素1(IL-1)ELISA試劑盒
雞免疫球蛋白G(IgG)ELISA試劑盒
雞血管活性腸肽(VIP)ELISA試劑盒
雞白細(xì)胞介素-10(IL-10)ELISA試劑盒
雞細(xì)胞間粘附分子-1(ICAM-1)ELISA試劑盒
雞白介素6(IL-6)ELISA試劑盒
雞白介素2(IL-2)ELISA試劑盒
雞細(xì)胞凋亡抑制因子ELISA試劑盒
雞皮質(zhì)酮(CS)ELISA試劑盒
雞α干擾素(IFN-α)ELISA試劑盒
雞轉(zhuǎn)化生長(zhǎng)因子β2(TGF-β2)ELISA試劑盒
雞胰高血糖素(GC)ELISA試劑盒
雞甲狀腺素(T4)ELISA試劑盒
雞三碘甲狀腺原氨酸(T3)ELISA試劑盒
雞抗肌內(nèi)膜抗體IgA(EMAIgA)ELISA試劑盒
雞核因子κB(NF-κB)ELISA試劑盒
雞弓形蟲循環(huán)抗原(TCA)ELISA試劑盒
雞甲狀旁腺激素(PTH)ELISA試劑盒
雞卵黃免疫球蛋白(IgY)ELISA試劑盒
雞蛋卵黃高磷蛋白磷酸肽(PPP)ELISA試劑盒
雞脂多糖/內(nèi)毒素(LPS)ELISA試劑盒
雞免疫球蛋白E(IgE)ELISA試劑盒
雞腫瘤壞死因子α(TNF-α)ELISA試劑盒
雞白介素1β(IL-1β)ELISA試劑盒
雞主要組織相容性復(fù)合體(MHC/B)ELISA試劑盒
雞熱休克蛋白70(HSP-70)ELISA試劑盒
雞白痢抗體檢測(cè)(PD)ELISA試劑盒
雞白介素18(IL-18)ELISA試劑盒
雞病毒性腸炎病毒(DEV)ELISA試劑盒
雞肌紅蛋白(MYO/MB)ELISA試劑盒
雞白介素9(IL-9)ELISA試劑盒
雞白介素3(IL-3)ELISA試劑盒
雞白血病抑制因子(LIF)ELISA試劑盒
雞轉(zhuǎn)化生長(zhǎng)因子β1(TGF-β1)ELISA試劑盒
雞C反應(yīng)蛋白(CRP)ELISA試劑盒
雞血管內(nèi)皮細(xì)胞生長(zhǎng)因子(VEGF)ELISA試劑盒
雞堿性成纖維細(xì)胞生長(zhǎng)因子(bFGF)ELISA試劑盒
雞可溶性細(xì)胞間粘附分子1(sICAM-1)ELISA試劑盒
雞可溶性E選擇素(sE-selectin)ELISA試劑盒
雞表皮生長(zhǎng)因子(EGF)ELISA試劑盒
雞胰島素樣生長(zhǎng)因子1(IGF-1)ELISA試劑盒
雞生長(zhǎng)激素(GH)ELISA試劑盒
雞乙酰乙酸檢測(cè)(ACAC)ELISA試劑盒
雞傳染性鼻炎抗體(IC)ELISA試劑盒
雞透明質(zhì)酸(HA)ELISA試劑盒
雞硫酸類肝素(HS)ELISA試劑盒
Switch, lid interlock with cable, RG6 9019375 qiagen
Cable, thermistor wiring, RG6 9019373 qiagen
Sensor, optics detector home, RG6 9019345 qiagen
Filter, fuse, switch main power, RG6 9019328 qiagen
IC, Microprocessor, state model SN, RG6 9019324 qiagen
Motor - Mixer, PyroQ24 9019239 qiagen
Mikrowin Agglutination Software 9234702 qiagen
Sealing ring, pressure relief valve, dis 9234691 qiagen
Plate, front, GSC 9020479 qiagen
Gripper module, PyroQ96MD 9019211 qiagen
Opto - Robot X motor , PyroQ96MD 9019202 qiagen
Buffer AW1 (151 ml) 1067924 qiagen
FMDV RT-PCR REAGENT 1061116 qiagen
Pump, SingleHead, Washer, (Dispensing) 9232898 qiagen
Teaching Tools, BR EZ1 9016668 qiagen
Cooling Block (M48) Adapter 9016590 qiagen
ZLV Tip Eject, RCS1 9016571 qiagen
Round Carrier Set (4), 12 x 15ml Tubes 9016518 qiagen
Carrier, tubes (1), sample track, Qsym 9019873 qiagen
PyroMark 1.0 OPC/IPC upgrade, PyroQ96ID 9019838 qiagen
Pipetting head, 1 ch. , Cx2 9019814 qiagen
Power supply, main, 24V, V2, QX 9019759 qiagen
Tip Adapter Kit, 300ul, BR8000 9013844 qiagen
Trough, 80 ml (10) 9013653 qiagen
Maintenance kit, semi annual, BR9604 9013409 qiagen
Plate holder for bucket 9238811 qiagen
Rotor-Gene Q User Manual 9019672 qiagen
O rings - VPT Fast connector, PyroVW 9019658 qiagen
Belt, Z-axis gearbox (black), 8 ch., Cx8 9019594 qiagen
Opto switch, Z-axis, 1 ch. , Cx2 9019577 qiagen
Cable, 8 core, axis motor assembly, CQx 9019564 qiagen
Plate Stacker A/B RD, RCS 9019537 qiagen
Vacuum trap, CX1 9019524 qiagen
PLATE SEPARATOR 96 WELL HIGH 9019506 qiagen
FastLane Cell Multiplex NR Kit (200) 216713 qiagen
QuantiFast Probe PCR Kit (80) 204252 qiagen
Board, distribution, no PTCs, RG3 9019404 qiagen
LED, blue (470), RG3 9019389 qiagen
Hinge assembly, front hinge plate, RG6 9019371 qiagen
Transformer, low volts, RG6 9019358 qiagen
AllStars Neg. siRNA AF 555 (20 nmol) 1027294 qiagen
LED, HRM, RG6 9019343 qiagen
Belt, toothed, src, detector optics, RG6 9019326 qiagen
Trough Lid Digene 9234807 qiagen
Board, shaker, non-CE 9234677 qiagen
Clamp, rubber, adapter plate, HS shaker, 9234466 qiagen
Ring, straining, pressure sensor 9233455 qiagen
Valve, dry way, auto vac, BR8000 9233443 qiagen
Valve - Dispense, PyroQ24 9019233 qiagen
Lead screw - Z robot, PyroQ96MD 9019218 qiagen
PCB 6 - Connection, PyroQ96MD 9019213 qiagen
Camera MX9, PyroQ96MD 9019187 qiagen
Buffer AVE + Azid 108 x (2/2),KG 1020953 qiagen
HSV Quantification Standards 1061096 qiagen
HBV Controls & Quantification Standards 1061119 qiagen
Software Pack. LiquiChip Policy Manager 9016743 qiagen
Transformer (230V), HS 9019867 qiagen
Riser block, 19.5 mm, QXT 9019865 qiagen
Power supply, high voltage, V2, QX 9019760 qiagen
Board, smart key, V2, QX 9019730 qiagen
Upgrade-Kit Shakerplatte autom BR8000 9013674 qiagen
Reagent holder, 5-trough 80 ml 9013610 qiagen
Maintenance kit, semi annual, BR9600 (B) 9013428 qiagen
Tubing set, with HSPS, BR9600 9013423 qiagen
Set Adjustment plate, Qsym 9019666 qiagen
Temperature Controller, EZ1xl 9019640 qiagen
Board, main assembly, HS 9019631 qiagen
O-ring, filter housing seal 2.5mm, CX1 9019601 qiagen
Power supply, QGX 9019586 qiagen
Slideout electrical tray (220V), C1x 9019583 qiagen
Sensor, detector home, RG3 9019429 qiagen
Motor, stepper no cable, detector, RG6 9019364 qiagen