Recombinant Human Interleukin-4 (IL-4)
Purified No Carrier Protein
(BCGF, BCDF, B Cell Stimulating Factor, BSF-1, MGC79402)
Prod. No.: I-186
Source E. coli
Recombinant Human Interleukin-4 （IL-4）
Purified No Carrier Protein
（BCGF, BCDF, B Cell Stimulating Factor, BSF-1, MGC79402）
Prod. No.: I-186
Source E. coli
Pkg. Size: 1 mg, 10 µg, 50 µg
Storage: -20°C to -70°C Detailed storage instructions below.
Description
Background:
Interleukin-4 （IL-4）, also known as B Cell Stimulating Factor, is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression （1）. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, which favor a humoral immune response while suppressing a cell mediated immune response controlled by Th1 cells （2）. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of IL-4. Another dominant function of IL-4 is inducing immunoglobulin class switching to the IgG1 and IgE isotypes in B cells, as well as, up-regulating MHC class II production （3）. IL-4 is the ligand for IL-4 receptor. The IL-4 receptor also binds to IL-13, which may contribute to overlapping functions with IL-13. IL-4 plays a dominant role in the development of allergic inflammation and asthma （4）.
Source
E. coli
Molecular Weight
The predicted molecular weight of Recombinant Human IL-4 is Mr 14 kDa.
State of Matter
Lyophilized
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline （1X PBS） pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Purity
>97% by SDS-PAGE and analyzed by silver stain.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Endotoxin
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-4 was determined by a cell proliferation assay using a human factor-dependent cell line, TF-1 （Kitamura, T. et al., 1989, J. Cell Physiol. 140:323）. The expected ED50 for this effect is typically 0.05 - 0.2 ng/ml.
Each investigator should determine their own optimal working dilution for specific applications.
Amino Acid Sequence
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Country of Origin
USA
References
1. Pike, J. W. et al. （2001） PNAS 98:2443
2. Finkelman, F. D. et al. （2003） J. Immunol. 170:3835
3. Paludan, S. R. et al. （1998） Scand. J. Immunol. 48:459
4. Ryan, J. J. et al. （2007） Crit. Rev. Immunol. 27:15
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