當前位置:杭州昊鑫生物科技股份有限公司>>云克隆>> SEA028Mu紅細胞生成素(EPO)檢測試劑盒
SEA028Mu
云克隆
代理商
杭州市
96T 2988元 10000盒可售
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更新時間:2024-07-11 15:00:18瀏覽次數(shù):149次
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級別 | 化工級 | 證書 | ISO系列證書 |
編號SEA028Mu
物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
實驗方法雙抗夾心
反應(yīng)時長3h
檢測范圍6.25-400pg/mL
靈敏度最小可檢測劑量小于等于2.68pg/mL.
樣本類型serum, plasma, tissue homogenates, cell lysates and other biological fluids
下載英文說明書 中文說明書
規(guī)格48T96T 96T*5 96T*10 96T*100
價格¥ 2988
本試劑盒用于檢測紅細胞生成素(EPO),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
分別于定值血清及血漿樣本中加入一定量的紅細胞生成素(EPO)(加標樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 84-102 | 89 |
EDTA plasma(n=5) | 92-101 | 95 |
heparin plasma(n=5) | 88-105 | 95 |
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
在定值血清及血漿樣本內(nèi)加入適量的紅細胞生成素(EPO),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中紅細胞生成素(EPO)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 89-97% | 91-99% | 96-105% | 78-94% |
EDTA plasma(n=5) | 82-102% | 92-99% | 82-91% | 86-97% |
heparin plasma(n=5) | 99-105% | 85-97% | 81-101% | 86-94% |
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
1. 實驗前標準品、試劑及樣本的準備;
2. 加樣(標準品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
將紅細胞生成素(EPO)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標準品或標本,其中的紅細胞生成素(EPO)與連接于固相載體上的抗體結(jié)合,然后加入生物su化的紅細胞生成素(EPO)抗體,將未結(jié)合的生物su化抗體洗凈后,加入HRP標記的親和素,再次洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的紅細胞生成素(EPO)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
雜志 | 參考文獻 |
Endocrinology | Prenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat Offspring [Endo: source] |
Journal of Ethnopharmacology | Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression [PubMed: 21333724] |
PLOS ONE | CD98 Positive Eosinophils Contribute to T Helper 1 Pattern Inflammation [PlosOne: Source] |
The Journal of Clinical Endocrinology & Metabolism | Alterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the Ovulation [PubMed: 22948762] |
Experimental & Molecular Medicine | Long-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expression [PubMed: PMC3509184] |
Biochem Biophys Res Commun. | Reevaluation of erythropoietin production by the nephron [Pubmed:24832733] |
Nutrition. | Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m. [Pubmed:2528040] |
J Appl Physiol (1985). | Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain Sickness [Pubmed:25324511] |
Expert Rev Proteomics | Plasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferrone [PubMed: 26496240] |
Oncotarget | KIAA0101 is associated with human renal cell carcinoma proliferation and migration induced by erythropoietin [pubmed:26575329] |
international journal of molecular sciences | Expression of Iron-Related Proteins Differentiate Non-Cancerous and Cancerous Breast Tumors. [pubmed:28216608] |
Veterinary Clinical Pathology | Occult gastrointestinal bleeding is a common finding in dogs with chronic kidney disease [pubmed:28186645] |
IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
Oxidative Medicine and Cellular Longevity | Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463] |
PLoS Neglected Tropical Diseases | Functional and phenotypic evaluation of eosinophils from patients with the acute form of paracoccidioidomycosis [pubmed:28489854] |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on duration of anoxia [S0197018617304783] |
Pesquisa Veterinária Brasileira | Relation between anaemia and bone marrow features and serum erythropoietin in dogs with chronic kidney disease [10.1590/s0100-736x2017000600011 ] |
Medical Journal Armed Forces India | Study of acute hypoxia markers in healthy subjects: Utility in post-crash investigation [10.1016/j.mjafi.2017.04.003] |
Neurochemistry International | Effects of erythropoietin on astrocytes and brain endothelial cells in primary culture during anoxia depend on simultaneous signaling by other cytokines and on … [Pubmed:29180303] |
Clinical and Experimental Medicine | Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502] |
Bulletin of Experimental Biology and Medicine | Signal Mechanism of the Protective Effect of Combined Preconditioning by Amtizole and Moderate Hypoxia [Pubmed:29308565] |
Bulletin of Experimental Biology and Medicine | Expression of Hif-1α, Nf-κb, and Vegf Genes in the Liver and Blood Serum Levels of HIF-1α, Erythropoietin, VEGF, TGF-β, 8-Isoprostane, and Corticosterone in Wistar Rats with High and Low Resistance to Hypoxia. Corticosterone in … [Pubmed: 30353332] |
FASEB JOURNAL | High-altitude hypoxia decreases plasma erythropoietin soluble receptor (sEpoR) concentration in humans [] |
Journal of Functional Foods | Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats [] |
博士論文 | PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE [] |
Nutrition | Effects of carbohydrate and glutamine supplementation on cytokine production by monocytes after exercise in hypoxia: a crossover, randomized and double-blind … [Pubmed: 31743809] |
BRITISH JOURNAL OF HAEMATOLOGY | Disordered serum erythroferrone and hepcidin levels as indicators of the spontaneous abortion occurrence during early pregnancy in humans [Pubmed: 32866306] |
JCI Insight. | Increased FGF-23 levels are linked to ineffective erythropoiesis and impaired bone mineralization in myelodysplastic syndromes [Pubmed: 32759495] |
Blood Cells, Molecules, and Diseases | EPAS1 regulates proliferation of erythroblasts in chronic mountain sickness [Pubmed: 32470757] |
Int J Endocrinol | miR663 Prevents Epo Inhibition Caused by TNF-Alpha in Normoxia and Hypoxia [34367277] |
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