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Forskolin試劑

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  • 型號(hào)

    HY-15371

  • 品牌

    MCE

  • 廠商性質(zhì)

    代理商

  • 所在地

    杭州市

規(guī)格

10 mM * 1 mL in DMSO 660元 10000可售

5 mg 600元 10000可售

10 mg 810元 10000可售

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CAS號(hào) 66575-29-9 產(chǎn)地 進(jìn)口
級(jí)別 其他
Forskolin試劑 是一種有效的腺苷酸環(huán)化酶 (adenylate cyclase) 激活劑，對于 I 型腺苷酸環(huán)化酶，IC50 為 41 nM，EC50 為 0.5 μM。Forskolin 也是一種細(xì)胞內(nèi) cAMP 形成的誘導(dǎo)劑。Forskolin 誘導(dǎo)多種細(xì)胞類型的分化并激活孕烷 X 受體 (PXR) 和 FXR。

注：本產(chǎn)品僅用于科研，不可用于臨床

Forskolin試劑簡介

Forskolin (Coleonol) 是一種有效的腺苷酸環(huán)化酶 (adenylate cyclase) 激活劑，對于 I 型腺苷酸環(huán)化酶，IC50 為 41 nM，EC50 為 0.5 μM。Forskolin 也是一種細(xì)胞內(nèi) cAMP 形成的誘導(dǎo)劑。Forskolin 誘導(dǎo)多種細(xì)胞類型的分化并激活孕烷 X 受體 (PXR) 和 FXR。Forskolin 對心臟產(chǎn)生正性肌力作用，并具有血小板抗凝集和降壓作用。Forskolin 也可誘導(dǎo)細(xì)胞自噬 (autophagy)。





Forskolin試劑生物活性

Forskolin (Coleonol) is a potent adenylate cyclase activator with an IC50 of 41 nM and an EC50 of 0.5 μM for type I adenylyl cyclase[1]. Forskolin is also an inducer of intracellular cAMP formation[2]. Forskolin induces differentiation of various cell types and activates pregnane X receptor (PXR) and FXR[3]. Forskolin exerts a inotropic effect on the heart, and has platelet antiaggregatory and antihypertensive actions. Forskolin also induces autophagy[4][5].

IC50 & Target

IC50: 41 nM (Adenylyl cyclase)[1]
EC50: 0.5 μM (Adenylyl cyclase)[1]

體外研究
(In Vitro)

Forskolin (Coleonol) is also a potent exosome biogenesis and/or secretion activator in prostate cancer (PC) cells[8].
Forskolin (Fsk) is a naturally occurring diterpene isolated from Coleus forskholii, directly activates adenylyl cyclase (AC) through its catalytic subunit to increase intracellular levels of cyclic adenosine monophosphate (cAMP)[1].
Forskolin (Fsk) affects the proliferation of the human T-cell lines such as Kit 225 and MT-2. Forskolin treatment inhibits the proliferation of both Kit 225 and MT-2 cells in a dose-dependent manner with an IC50 equal to ~5 μM Fsk. Forskolin treatment (10-100 μM) increases cAMPi levels ~5- to 20-fold above basal levels, which reache maximum levels between 50-100 μM Forskolin[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

體內(nèi)研究
(In Vivo)

The Forskolin (Coleonol)-treated Mrp4-/- mice shows an increased number of Ki67-positive and cleaved caspase 3-positive ECs, a significant decrease in the amount of pericyte coverage, and a reduced number of empty sleeves. In pups exposed to hyperoxia (75% oxygen) from P7 to P12, the Mrp4-/- mice shows a significant increase in the unvascularized retinal area[2].
The average blood glucose in the healthy rat group is 102.12±1.94 mg/dL, 101.25±3.56 for control group and 103±2.08 in forskolin group. The data shows that glucose levels at the end of the study are lower in forskolin group, with a significant difference according to the statistical tests applied (p=0.03)[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

410.50

性狀

Solid

Formula

C22H34O7

CAS 號(hào)

66575-29-9

中文名稱

毛喉素

結(jié)構(gòu)分類

  • Terpenoids

  • Diterpenoids

來源

  • Plants

  • Compositae

  • Gochnatia polymorpha (Less) Cabr.

  • Plants

  • Labiatae

運(yùn)輸條件

Room temperature in continental US; may vary elsewhere.

儲(chǔ)存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性數(shù)據(jù)
In Vitro:

DMSO : 100 mg/mL (243.61 mM; Need ultrasonic)

配制儲(chǔ)備液
濃度溶劑體積質(zhì)量1 mg5 mg10 mg
1 mM2.4361 mL12.1803 mL24.3605 mL
5 mM0.4872 mL2.4361 mL4.8721 mL
10 mM0.2436 mL1.2180 mL2.4361 mL
*

請根據(jù)產(chǎn)品在不同溶劑中的溶解度選擇合適的溶劑配制儲(chǔ)備液；一旦配成溶液，請分裝保存，避免反復(fù)凍融造成的產(chǎn)品失效。
儲(chǔ)備液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 儲(chǔ)存時(shí)，請?jiān)?6 個(gè)月內(nèi)使用，-20°C 儲(chǔ)存時(shí)，請?jiān)?1 個(gè)月內(nèi)使用。

In Vivo:

請根據(jù)您的實(shí)驗(yàn)動(dòng)物和給藥方式選擇適當(dāng)?shù)娜芙夥桨?。以下溶解方案都請先按?In Vitro 方式配制澄清的儲(chǔ)備液，再依次添加助溶劑：

——為保證實(shí)驗(yàn)結(jié)果的可靠性，澄清的儲(chǔ)備液可以根據(jù)儲(chǔ)存條件，適當(dāng)保存；體內(nèi)實(shí)驗(yàn)的工作液，建議您現(xiàn)用現(xiàn)配，當(dāng)天使用； 以下溶劑前顯示的百
分比是指該溶劑在您配制終溶液中的體積占比；如在配制過程中出現(xiàn)沉淀、析出現(xiàn)象，可以通過加熱和/或超聲的方式助溶

  • 1.


    請依序添加每種溶劑： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution


  • 2.


    請依序添加每種溶劑： 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.09 mM); Clear solution






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