杭州昊鑫生物科技股份有限公司
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EASYspin Plus 植物RNA快速提取試劑盒

參  考  價:910
具體成交價以合同協(xié)議為準
  • 型號

    RN3802

  • 品牌

    艾德萊

  • 廠商性質(zhì)

    代理商

  • 所在地

    杭州市

規(guī)格

50次 910元 100000瓶可售

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更新時間:2024-06-19 17:45:11瀏覽次數(shù):273次

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產(chǎn)地 國產(chǎn) 規(guī)格 50 次
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RN38-EASYspin Plus 植物RNA快速提取試劑盒
適用于快速提取植物組織細胞總RNA,使用基因組DNA清除柱技術可有效清除電泳可見gDNA殘留,RNA可用于反轉(zhuǎn)錄PCR,熒光定量PCR等。


RN38-EASYspin Plus 植物RNA快速提取試劑盒  

注:本產(chǎn)品僅用于科研,不可用于臨床

貨號#
規(guī)格
價格
RN3802
50
910


產(chǎn)品介紹

    本公司推出EASYspin無苯fen、氯仿RNA快速提取技術基礎上,又成功研發(fā)基因組DNA清除柱技術可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。裂解液迅速裂解細胞和滅活細胞RNA酶,植物RNA助提劑PLANTaid幫助結(jié)合多糖多酚并通過離心去除,然后裂解混合物用乙醇調(diào)節(jié)RNA結(jié)合吸附到基因組DNA清除柱,基因組DNA清除柱子同時吸附清除殘留的DNA, 然后RNA被選擇性洗脫濾過。濾過的RNA用乙醇調(diào)節(jié)結(jié)合條件后,RNA在高離序鹽狀態(tài)下選擇性吸附于離心柱內(nèi)硅基質(zhì)膜, 再通過一系列快速的漂洗-離心的步驟, 去蛋白液和漂洗液將細胞代謝物,蛋白等雜質(zhì)去除, 最后低鹽的RNase free H20將純凈RNA從硅基質(zhì)膜上洗脫。

產(chǎn)品特點:

1.不使用有毒的苯fen,氯仿等試劑,也不需要乙醇沉淀等步驟。
2.簡捷,單個樣品操作一般可在25分鐘內(nèi)完成,簡單快速的試劑盒。
3.植物RNA助提劑可以有效結(jié)合多糖多酚,提高清除效果。
4.成功研發(fā)基因組DNA清除柱技術可以有效清除gDNA殘留,得到的RNA一般不需要DNase消化,可用于反轉(zhuǎn)錄PCR、熒光定量PCR等實驗。
5.適應性極其廣泛,可以提取包括棉花、松針、冬青樹葉、葡萄葉片、等100多種國內(nèi)外試劑盒提取失敗的樣品。詳細樣品列表請參考公司主頁產(chǎn)品介紹。
6.多次柱漂洗確保高純度,OD260/OD280典型的比值達1.9~2.2,基本無DNA殘留,可用于RT-PCR,Northern-blot和各種實驗。


使用RN38-EASYspin Plus 植物RNA快速提取試劑盒  發(fā)表的部分文獻?。?!

1.     桃果實、花、根、葉:Isolation, characterisation and phylogenetic analysis of resistance gene    analogues in a wild species of peach (Prunus kansuensis).Canadian Journal of Plant Science,    2011, 91(6): 961-970


2.     櫻桃花、葉、顎等各部位:Over-expression of the PaAP1 gene from sweet cherry (Prunus avium    L.)    causes early floweri.Journal of Plant Physiology, 2012,Available online 1 December    2012


3.     洋蔥根、莖、蕾、葉、雌雄蕊等各部位:Cloning and Expression Analysis of A Putative B Class MADS-box    Gene of AcPI in Onion. Scientia Agricultura Sinica, 2012, 45(23):4759-4769


4.     蕪菁:Isolation and Functional Characterisation of the Genes Encoding Δ8-Sphingolipid       Desaturase from Brassica rapa. Journal of Genetics and Genomics Volume 39, Issue 1, January    2012,    Pages 47–59


5.     蕪  菁 1 :EXPRESSION, DIVERGENCE AND EVOLUTION OF THE CALEOSIN GENE FAMILY IN BRASSICA RAPA. Arch. Biol. Sci., Belgrade, 65 (3), 863-876, 2013 DOI:10.2298/ABS1303863H


6.     番茄葉:Effect of Low Temperature Stress on the Expression of ProDH Gene and the Activities of the Proline Dehydrogenase in Leaves of Tomato Seedling. Chinese Agricultural Science  Bulletin 2012,28(10):132-135


7.     梔子葉:Isolation of High Quality Total RNA from Gardenia jasminoides Eills.Chinese Agricultural    Science Bulletin.2012, 28(27):194-198


8.     油桐果實:Cui Qinqin, Han Xiaojiao, Chen Yicun, Zhan Zhiyong, Lin Liyuan, Wang Yangdong.     Isolation and Expression Characteristics of Biotin Carboxyl Carrier Protein Coding Gene(VfBCCP)  from Vernicia fordii.SCIENTIA SILVAE SINICAE. 2012, 48(8): Available online August


9.     油桐果實1:Selection of Reliable Reference Genes for Gene Expression Studies Using Real-Time    PCR in Tung Tree during Seed Development. PLoS ONE, 2012, 7(8): e43084


10. 紫菜:Molecular cloning and expression analysis of ribosomal protein S7 gene from Porphyra    haitanensis. JOURNAL OF FISHERIES OF CHINA, 2011, 35(12):1814-1821


11. 石斛:Molecular characterization of a mitogen-activated protein kinase gene DoMPK1 in Dendrobium officinale. Acta Pharmaceutica Sinica, 2012, 47 (12): 1703-1709


12. 石斛1:ESTs Analysis Reveals Putative Genes Involved in Symbiotic Seed Germination in Dendrobium officinale. Symbiotic Germination Genes in D. officinale. August 2013 | Volume 8 | Issue 8 | e72705


13. 大豆:RNA-seq Analysis Reveals Ethylene-Mediated Reproductive Organ Development and Abscission in Soybean(Glycine max L. Merr.). Plant Mol Biol Rep, 2012, published online: 4 Dec, 2012


14. 大豆1:Construction of ethylene regulatory network based on the phytohormones related gene transcriptome profiling and prediction of transcription factor activities in soybean. Acta Physiol Plant, 2012, published online: 12 Dec, 2012


15. 紅花玉蘭:Expression Analysis of MAwuAG in Different Organs and Developmental Stages of Magnolia wufengensis. Chinese Bulletin of Botany, 2013, 48 (2): 1–5


16. 毛桃:Cloning and Phylogeny Analysis of PpAP2 Floral Homologous Genes in Peach. Chinese Agricultural Science Bulletin, 2013, 29(7): 99-104


17. 五倍子:Cloning and characterisation of a phenylalanine ammonia-lyase gene from Rhus chinensis. Plant Cell Rep, 2013, published online:15 March, 2013


18. :五倍子1:Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis.J. Plant Biochem. Biotechnol. DOI 10.1007/s13562-013-0231-9


19. 青杄 :cDNA Cloning and Bioinformatic Analysis of the sPPa1 Gene form Picea wilsonii. Plant Science Journal, 2012, 30(40): 394-401


20. 青杄 1:cDNA Cloning and Bioinformatic Analysis of PsbO Gene from Picea wilsonii.Life Science Research, 2012, 16(3): 201-206


21. 青杄 2:Cloning and Tissue Expression Analysis of PwPSAF in Picea wilsonii. SCIENTIA SILVAE SINICAE. Vol. 49,No. 10, Oct. 2013.


22. 洋蔥:Molecular Cloning and Transcriptional Analysis of the Putative AGAMOUS Homolog AcAG in Onion (Allium cepa. Plant Mol Biol Rep, DOI 10.1007/s11105-013-0607-y


23. 木瓜:XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds. JOURNAL ARTICLE. 2013-6-17.


24. 木瓜1:Two novel diacylglycerol acyltransferase genes from Xanthoceras 2 sorbifolia are responsible for its seed oil content. GENE-38688; No. of pages: 9; 4C:


25. 柑橘:Efficient auto-excision of a selectable marker gene from transgenic citrus by combining the Cre/loxP system and ipt selection. Plant Cell Rep, DOI 10.1007/s00299-013-1470-x


26. 柑橘1:Expression Analysis of Three Phloem-specific Promoters in Transgenic Poncirus trifoliata. Acta Horticulturae Sinica. 2014, 41(1): 1–8.


27. 柑橘2: Activation of three pathogen-inducible promoters in transgenic citrus (Citrus sinensis Osbeck) after Xanthomonas axonopodis pv. citri infection and wounding. Plant Cell Tiss Organ Cult. DOI 10.1007/s11240-013-0423-y.


28. 茶梅花瓣:Comparison and Analysis of Methods of Extracting Total RNA from Petals of Camellia sasanqua. Chinese Agricultural Science Bulletin.2013,29(28):129-133.


29. 梔子:Isolation of High Quality Total RNA fromGardenia jasminoides Eills. Chinese Agricultural Science Bulletin. 2012, 28(27):194-198


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注:本產(chǎn)品僅用于科研,不可用于臨床


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