人蛋白酪氨酸磷酸酶(PTP/PTPase/CD148)ELISA 檢測試劑盒,人蛋白酪氨酸磷酸酶(PTP/PTPase/CD148)ELISA 檢測試劑盒
人蛋白酪氨酸磷酸酶(PTP/PTPase/CD148)ELISA 檢測試劑盒*試驗原理:
ZR試劑盒是固相夾心法酶聯(lián)免疫吸附實驗(ELISA).已知ZR濃度的標(biāo)準(zhǔn)品、未知濃度的樣品加入微孔酶標(biāo)板內(nèi)進(jìn)行檢測。先將ZR和生物素標(biāo)記的抗體同時溫育。洗滌后,加入親和素標(biāo)記過的HRP。再經(jīng)過溫育和洗滌,去除未結(jié)合的酶結(jié)合物,然后加入底物A、B,和酶結(jié)合物同時作用。產(chǎn)生顏色。顏色的深淺和樣品中ZR的濃度呈比例關(guān)系。
試劑盒內(nèi)容及其配制
試劑盒成份(2-8℃保存)
96孔配置
48孔配置
配制
96/48人份酶標(biāo)板
1塊板(96T)
半塊板(48T)
即用型
塑料膜板蓋
1塊
半塊
即用型
標(biāo)準(zhǔn)品:40nmol/L
1瓶(0.6ml)
1瓶(0.3ml)
按說明書進(jìn)行稀稀
空白對照
1瓶(1.0ml)
1瓶(0.5ml)
即用型
標(biāo)準(zhǔn)品稀釋緩沖液
1瓶(5ml)
1瓶(2.5ml)
即用型
生物素標(biāo)記的抗ZR抗體
1瓶(6ml)
1瓶(3.0ml)
即用型
親和鏈酶素-HRP
1瓶(10ml)
1瓶(5.0ml)
即用型
洗滌緩沖液
1瓶(20ml)
1瓶(10ml)
按說明書進(jìn)行稀釋
底物A
1瓶(6.0ml)
1瓶(3.0ml)
即用型
底物B
1瓶(6.0ml)
1瓶(3.0ml)
即用型
終止液
1瓶(6.0ml)
1瓶(3.0ml)
即用型
標(biāo)本稀釋液
1瓶(12ml)
1瓶(6.0ml)
即用型
【簡單介紹】
人蛋白酪氨酸磷酸酶(PTP/PTPase/CD148)ELISA試劑盒"標(biāo)本取樣的基本要求 :
*標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實驗。若不能馬上進(jìn)行試驗,可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
*不能檢測含NaN3的樣品,因NaN3抑制辣根過氧化物酶的(HRP)活性。
RS12627B 豬血栓調(diào)節(jié)蛋白(TM)elisa試劑盒 PoFAine thrombomodulin,TM Elisa試劑盒
RS12628B 豬血小板衍生生長因子(PDGF)elisa試劑盒 PoFAine plaet-derived growth factor,PDGF Elisa試劑盒
RS12629B 豬氧化低密度脂蛋白(OxLDL)elisa試劑盒 PoFAine oxidized lowdensity lipoprotein,OxLDL Elisa試劑盒
RS12630B 豬組織型纖溶酶原激活劑(t-PA)elisa試劑盒 PoFAine Tissue-type Plasiminogen Actilyse,t-PA Elisa試劑盒
RS12631B 豬高鐵血紅蛋白(MHB)elisa試劑盒 PoFAine Methemoglobin,MHB Elisa試劑盒
RS12632B 豬Ⅲ型前膠原氨基端肽(PⅢNT)elisa試劑盒 PoFAine procollagen Ⅲ N-terminal peptide,PⅢNT Elisa試劑盒
RS12633B 豬單核細(xì)胞增多性李斯特菌素(listeriolysin)elisa試劑盒 PoFAine listeriolysin Elisa試劑盒
RS12634B 雞主要組織相容性復(fù)合體(MHC/B)elisa試劑盒 Chicken major histocompatibility complex,MHC/B Elisa試劑盒
RS12635B 雞熱休克蛋白70(HSP-70)elisa試劑盒 Chicken Heat Shock Protein 70,Hsp-70 Elisa試劑盒
RS12636B 雞白痢抗體檢測(PD)elisa試劑盒 Chiken Pullorum Diseaes,PD Elisa試劑盒
RS12637B 雞分泌型免疫球蛋白A(SIgA)elisa試劑盒 Chicken secretory immunoglobulin A,SIgA Elisa試劑盒
RS12638B 雞α干擾素(IFN-α)elisa試劑盒 Chicken Interferon α,IFN-α Elisa試劑盒
RS12639B 雞白介素18(IL-18)elisa試劑盒 Chicken Interleukin 18,IL-18 Elisa試劑盒
RS12640B 雞白介素1(IL-1)elisa試劑盒 Chicken Interleukin 1,IL-1 Elisa試劑盒
RS12641B 雞病毒性腸炎病毒(DEV)elisa試劑盒 Chicken virus enteritis,DEV Elisa試劑盒
RS12642B 雞肌紅蛋白(MYO/MB) elisa試劑盒 Chicken Myoglobin,MYO/MB Elisa試劑盒
RS12643B 雞白介素9(IL-9)elisa試劑盒 Chicken Interleukin 9,IL-9 Elisa試劑盒
RS12644B 雞白介素3(IL-3)elisa試劑盒 Chicken Interleukin 3,IL-3 Elisa試劑盒
RS12645B 雞白血病抑制因子(LIF)elisa試劑盒 Chicken Leukemia inhibitory factor,LIF Elisa試劑盒
RS12646B 雞轉(zhuǎn)化生長因子β1(TGF-β1)elisa試劑盒 Chicken Transforming Growth factor β1,TGF-β1 Elisa試劑盒
RS12647B 雞C反應(yīng)蛋白(CRP)elisa試劑盒 Chicken C-Reactive Protein,CRP Elisa試劑盒
RS12648B 雞血管內(nèi)皮細(xì)胞生長因子(VEGF)elisa試劑盒 Chicken Vascular Endothelial cell Growth Factor,VEGF Elisa試劑盒
RS12649B 雞免疫球蛋白G(IgG)elisa試劑盒 Chicken Immunoglobulin G,IgG Elisa試劑盒
RS12650B 雞堿性成纖維細(xì)胞生長因子(bFGF)elisa試劑盒 Chicken basic fibroblast growth factor,bFGF Elisa試劑盒
RS12651B 雞可溶性細(xì)胞間粘附分子1(sICAM-1)elisa試劑盒 Chicken Soluble InteFAellular Adhesion Molecule-1,sICAM-1 Elisa試劑盒
RS12652B 雞可溶性E選擇素(sE-selectin)elisa試劑盒 Chicken soluble E-selectin,sE-selectin Elisa試劑盒
RS12653B 雞表皮生長因子(EGF)elisa試劑盒 Chicken Epidermal growth factor,EGF Elisa試劑盒
RS12654B 雞胰島素樣生長因子1(IGF-1)elisa試劑盒 Chicken Insulin-like growth factors 1,IGF-1 Elisa試劑盒 ab25634 Mouse monoclonal [143-30] to CD55 (FITC) 1ml 3450
ab25635 Mouse monoclonal [143-30] to CD55 (Biotin) 1ml 3450
ab25636 Mouse monoclonal [RVS10] to Transferrin Receptor (FITC) 1ml 3450
ab25637 Mouse monoclonal [RVS10] to Transferrin Receptor (Biotin) 1ml 3450
ab25638 Mouse monoclonal [DX3] to CD95 100ug 3450
ab25639 Rat monoclonal [2B8] to c-Kit (Phycoerythrin) 100ug 3450
ab2564 Rabbit polyclonal to RGS3 50ug 3450
ab25640 Mouse monoclonal [MEM 188] to NCAM (Biotin) 1ml 3450
ab25642 Hamster monoclonal [PV-1] to CD28 (Phycoerythrin) 100ug 3450
ab25644 Rat monoclonal [390] to CD31 (Phycoerythrin) 100ug 3450
ab25645 Rat monoclonal [B19.1] to CD5 (Biotin) 100ug 3450
ab25646 Rat monoclonal [91] to CD24 (Phycoerythrin) 100ug 3450
ab25647 Rat monoclonal [C363.16A] to CD45RB (Phycoerythrin) 100ug 3450
ab25648 Rat monoclonal [4H8E6] to CD5 (FITC) 100ug 3450
ab25649 Armenian Hamster monoclonal [H57-597] to TCR alpha + TCR beta (Phycoerythrin) 100ug 3450
ab2565 Rabbit polyclonal to MEK3 50ug 3450
ab25650 Rabbit polyclonal to beta Synuclein 100ul 3450
ab25651 Mouse monoclonal [RVS10] to Transferrin Receptor - BSA and Azide free 100ug 3450
ab25652 Mouse monoclonal [SB70] to ZAP70 (HRP) 1ml 3450
ab25653 Mouse monoclonal [1B5] to HHV4 100ul 3450
ab25656 Rat monoclonal [2D6] to CD22 (Biotin) 100ug 3450
ab25657 Rat monoclonal [91] to CD24 (FITC) 100ug 3450
ab25658 Rat monoclonal [91] to CD24 100ug 3450
ab25659 Rat monoclonal [GK1.5] to CD4 (FITC) 100ug 3450
ab2566 Rabbit polyclonal to APRIL 50ug 3450
ab25660 Rat monoclonal [C363.29B (YCD3-1)] to CD3 epsilon (FITC) 100ug 3450
ab25661 Rat monoclonal [GL24] to CD45RC (FLMA) 100ug 3450
ab25662 Hamster monoclonal [PV-1] to CD28 (Allophycocyanin) 50ug 3450
ab25663 Armenian Hamster monoclonal [UC7-13D5] to TCR gamma + TCR delta (Phycoerythrin) 100ug 3450
ab25664 Mouse monoclonal [2H11] to UEV2 200ul 3450
ab25665 Armenian Hamster monoclonal [145-2C11] to CD3 epsilon (Phycoerythrin) 100ug 3450
zui重要是安全性
1. 避免直接接觸終止液和底物A、B。一旦接觸到這些液體,請盡快用水沖洗。
2. 實驗中不要吃喝、抽煙或使用化妝品。
3. 不要用嘴吸取試劑盒里的任何成份。
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