骨橋素(OPN)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Osteopontin (OPN) 48T/96T Y16120 Bos taurus; Bovine (Cattle)
天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Aspartate Aminotransferase (AST) 48T/96T Y16121 Bos taurus; Bovine (Cattle)
蛋白激酶Bβ(PKBβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法) ELISA Kit for Protein Kinase B Beta (PKBb) 豬內(nèi)皮型一氧化氮合成酶(eNOS)ELISA檢測試劑盒48T/96T Y16122 Rattus norvegicus (Rat)
性 狀:盒裝液體
保存溫度: 2~8℃
有 效 期:6個月 (six month)
酶標包被板:96孔/48孔(48T酶標包被板是96T一半)
實驗原理:ELISA的基礎(chǔ)是抗原或抗體的固相化及抗原或抗體的酶標記。結(jié)合在固相載體表面的抗原或抗體仍保持其免疫學活性,酶標記的抗原或抗體既保留其免疫學活性,又保留酶的活性。在測定時,受檢標本(測定其中的抗體或抗原)與固相載體表面的抗原或抗體起反應。用洗滌的方法使固相載體上形成的抗原抗體復合物與液體中的其他物質(zhì)分開。再加入酶標記的抗原或抗體,也通過反應而結(jié)合在固相載體上。此時固相上的酶量與標本中受檢物質(zhì)的量呈一定的比例。加入酶反應的底物后,底物被酶催化成為有色產(chǎn)物,產(chǎn)物的量與標本中受檢物質(zhì)的量直接相關(guān),故可根據(jù)呈色的深淺進行定性或定量分析。
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