Qiagen 52906 RNA病毒提取試劑盒
操作步驟:應(yīng)用QIAGEN Plasmid Kits純化過程中,通過離心取出細菌裂解液。然后將澄清的裂解液裝入陰離子交換柱,在適當(dāng)?shù)牡望}濃度和pH值條件下,陰離子交換柱會選擇性地結(jié)合質(zhì)粒DNA。RNA、蛋白質(zhì)、代謝物和其他小分子雜質(zhì)通過中鹽濃度洗滌去除,應(yīng)用高鹽濃度緩沖夜洗脫超純的質(zhì)粒DNA(見流程圖"Comparison of QIAGEN Plasmid Kit Procedures")。通過異丙醇沉淀濃縮并脫鹽DNA,離心法收集DNA。
應(yīng)用
包含離心柱、緩沖液和收集管,用于純化100 bp以上的PCR DNA PIAN段。 通過快速、方便的結(jié)合-洗滌-洗脫步驟純化10 kb以內(nèi)的DNA PIAN段,洗脫體積為30-50 μl。內(nèi)置的pH指示劑可簡單確定DNA結(jié)合到離心柱的*pH值,整個過程可在 QIAcube上自動化。
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