FAM hydrazide,酰肼綠色熒光染料
產品介紹
FAM hydrazide, 5-isomer
Many natural and synthetic molecules contain aldehyde or ketone carbonyl groups. These carbonyls react with hydrazides with the formation of hydrazones. The reaction is spontaneous at pH values around neutral, and the resulting hydrazones are very stable.
pounds containing 1,2-diol function, like sugars, can be oxidized with sodium periodate with the formation of carbonyl pounds for the subseuent modification with hydrazides. This is an efficient method for the labeling of glycoproteins (like antibodies), and polysaccharides.
FAM hydrazide is a hydrazide label for the attachment of fluorescein, a bright dye for 488 nm channel, to carbonyl pounds.
General properties
Appearance: | yellow solid |
Mass spec M+ increment: | 372.07 |
Molecular weight: | 426.81 |
Molecular formula: | C21H15N2ClO6 |
Solubility: | good in ethanol, DMF, DMSO |
uality cont*ol: | NMR 1H, HPLC-MS (95%) |
Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
Spectral properties
Excitation maximum, nm: | 494 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 520 |
Fluorescence uantum yield: | 0.9 |
CF260: | 0.20 |
CF280: | 0.17 |
結構式：
FAM hydrazide,酰肼綠色熒光染料
西安凱新生物科技有限公司
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