官方微信|手機(jī)版|本站服務(wù)|買家中心|行業(yè)動態(tài)|幫助

產(chǎn)品|公司|采購|招標(biāo)

衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒

參考價面議
具體成交價以合同協(xié)議為準(zhǔn)
  • 公司名稱齊一生物科技(上海)有限公司
  • 品       牌
  • 型       號
  • 所  在  地上海市
  • 廠商性質(zhì)生產(chǎn)廠家
  • 更新時間2015/7/22 16:18:44
  • 訪問次數(shù)1939
在線詢價 收藏產(chǎn)品 進(jìn)入商鋪 同類產(chǎn)品

聯(lián)系方式:程成 查看聯(lián)系方式

該公司近期關(guān)注度420643

聯(lián)系我們時請說明是 制藥網(wǎng) 上看到的信息,謝謝!

齊一生物主要覆蓋于免疫學(xué),分子生物學(xué),病理學(xué),生物化學(xué)細(xì)胞生物學(xué)生命科學(xué)的各個領(lǐng)域。已迅速發(fā)展成為國內(nèi)科學(xué)試劑的運(yùn)營商之一,是國家重點(diǎn)實(shí)驗(yàn)室*供應(yīng)商,并與國內(nèi)多家科研單位緊密合作。

齊一生物致力于檢測試劑盒的自主研發(fā)、生產(chǎn)和銷售以及樣本檢測等技術(shù)服務(wù)經(jīng)營為一體的專業(yè)化生物工程技術(shù)公司!為廣大科研工作者、企業(yè)和校提供質(zhì)優(yōu)價廉的實(shí)驗(yàn)室用品


齊一生物長期經(jīng)營ELISA試劑盒,進(jìn)口ELISA試劑盒及抗體(免費(fèi)代測),分子學(xué)生物試劑盒,金標(biāo)法檢測試劑盒,比色法檢測試劑盒,細(xì)胞菌株,培養(yǎng)基,細(xì)胞因子,生化試劑,耗材等生物試劑產(chǎn)品
衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒價格:全國:,到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國產(chǎn)ELISA試劑盒,性價比*
衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒 產(chǎn)品信息

衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒價格:全國:,到我司741653262,齊一生物科技(上海)有限公司提供大量品牌進(jìn)口原裝,分裝,以及穩(wěn)定性強(qiáng)的國產(chǎn)ELISA試劑盒,性價比*

衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒操作步驟:
1.包被過程(注意設(shè)置空白對照,陰性對照):
將所用抗原用包被稀釋液稀釋到適當(dāng)濃度(一般所需抗原包被量為每孔20-200μg),每孔抗原加入100μl,置37℃,4h,或4℃,24h;棄去孔中液體.(為避免蒸發(fā),板上應(yīng)加蓋或?qū)迤椒旁诘撞坑袧窦啿嫉慕饘贊窈兄?
2.封閉酶標(biāo)反應(yīng)孔:
5%小牛血清置37℃封閉40min.封閉時將封閉液加滿各反應(yīng)孔,并去除各孔中的氣泡,封閉結(jié)束后用洗滌液滿孔洗滌3遍,每遍3min.
洗滌方法:吸干孔內(nèi)反應(yīng)液,將洗滌液注滿板孔,放置2min略作搖動,吸干孔內(nèi)液,傾去液體后在吸水紙上拍干
洗滌次數(shù)3次
3.加入待檢測樣品(建立合適的濃度梯度):
檢測時一般采用1:50-1:400的稀釋度,應(yīng)采用較大稀釋體積進(jìn)行,一般保證樣品吸取量>20μl.
將稀釋好的樣品加入酶標(biāo)反應(yīng)孔中,每樣品少加雙孔,每孔100μl,置于37℃,40-60min.用洗滌液滿孔洗滌3遍,每遍3min.
4.加入酶標(biāo)抗體:
酶標(biāo)抗體:根據(jù)酶結(jié)合物提供商提供的參考工作稀釋度進(jìn)行.37℃,30-60min之間.短于30min往往結(jié)果不穩(wěn)定.每孔加100μl.洗滌同前.
5.加入底物液(現(xiàn)用現(xiàn)配):
*TMB-過氧化氫尿素溶液,OPD-過氧化氫底物液系統(tǒng)次之.
底物加入量:每孔100μl,置37℃避光放置3-5分鐘,加入終止液顯色.
6.終止反應(yīng):
每孔加入終止液50μl終止反應(yīng),于20min內(nèi)測定實(shí)驗(yàn)結(jié)果.
7.結(jié)果判斷:
OPD顯色后采用492nm波長,TMB反應(yīng)產(chǎn)物檢測需要450nm波長.檢測時一定要首先進(jìn)行空白孔系統(tǒng)調(diào)零.用測定標(biāo)本孔的吸收值與一組陰性標(biāo)本測定孔平均值的比值(P/N)表示,當(dāng)P/N大于2時作為抗體的效價.(數(shù)值的大小依具體檢測要求而定.)

9019168    Set, O-Ring (32), Qsym
9014168    RCS - Shipping Crate
9021250    Tube Spinner Assembly, DCU
9021189    Adapter, skirted PCR plate, BS96
9021023    Lid, waste box, SP, Qsym
9021014    Controller, Gripper, VR, DCU
9021010    Controller, X Axis, VR, DCU
9020904    Lid, SMC, QXT
9020901    Multi.Carrier (2) for 7 cult.tubes 50ml
9020709    Cable, y, z, & p motor harness, C34
9020645    Waste chute, bent, AS, Cabinet Qsym
9020558    Tubing, plete vacuum assembly, CX1
9020485    Stepper drive assembly, GSC
9020320    PM kit, C12 & QIAgility (HEPA), CG1
9017624    Drive tooth wheel, y-drive, BR8RD V2.1
9017601    Wash Pump, BR8 RD
9020260    Front door, Qsym
9020259    Side Pannel, top right, Qsym
9017267    SOW QIAsoft  MDx Dispens. RNase-free.PRO
9017258    BR 8000, Plasmid Purification Package
9020223    PCB, USB, QXT
9020220    Cover, Pipettor, 1 ch. (silver), QGY
972022    PyroMark Q96 CpG MLH1 (96)    For quantification of methylation level in MLH1 promoter
133226    NeXtalStock Sodium cacodyl. pH 5.0 (100)
301307    SuperFect Transfection Reagent(4x1.2 ml)    For 160 transfections in 60 mm dishes or 640 transfections in 12-well plates
56404    QIAamp DNA FFPE Tissue Kit (50)
133210    NeXtalStock MES pH 7.0 (100)
133200    NeXtalStock Imidazole pH 6.0 (200)
293033    mericon Apricot kernels (24)
292075    mericon Ruminant Kit (96)
9012656    Cable, pressure sensor, BR8000
9012599    Disposable Filter-Tips, 300μl (960)    Conducting disposable filter-tips
9001941    QIAxcel Advanced
205920    QIAGEN LongRange 2Step RT-PCR Kit (20)    "For 20 PCR rxns a 50 μl: 
cDNA Step, 10 RT rxns a 20 μl: LongRange RT enzyme, LongRange RT reaction buffer, dNTP′s, Oligo dT, RNAse free water
PCR Step: QIAGEN LongRange PCR Kit (20)"
205310    QuantiTect Rev.Transcription Kit (10)
291063    mericon GMO Screen bar (24)
9001920    PyroMark Q96 MD Automated, PrioPLUS    "PyroMark Q96 MD Automated Instrument 
Installation ofPyroMark Q96 MD Automated & Introductory training 
Warranty extension to 3 yrs
On site service, 48 hr response
Preventive Maintenance (1 onsite PM per year)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
133117    NeXtalStock Succinic acid salt (200)
133110    NeXtalStock Sodium phosphate (200)
133194    NeXtalStock di-Sodium phosphate (200)
9015515    Magnet unit, plete
9018451    Piston, Anode (1), QX
9015436    Handler with grippers, BR8000
9015403    Belt, D-timing, M96
9001862    Rotor-Gene Q 2plex (PC) PrioPLUS    "Rotor-Gene Instrument 
Installation of Rotor-Gene & Introductory training 
Warranty extension to 3 yrs
Loaner available in 2 days*
Annual Inspections (1 onsite PM per yearr)
Priority service provision over non-contract customers
Documented installation & PM report
Unlimited number of repair service
All cost covered (travel, labor, parts)"
9012377    Holder, liquid bottle, BR8000
9012308    Modul, Washstation, P3xxx
1067932    Buffer RBC (45 ml)
1067928    Buffer FG2 (750 ml)
133083    NeXtalStock PEG 3,350 (200)
204656    QuantiFast Multiplex PCR Kit (2000)
204654    QuantiFast Multiplex PCR Kit (400)
805945    REPLI-g Service (500 μg)
290033    mericon Campylobacter spp Kit (24)
204354    QuantiFast Probe PCR+ROXVial Kit (400)
133045    NeXtalStock EDTA pH 8.0 (200)
133044    NeXtalStock Ethyl. imine polymer (200)
9018400    Cable, assy, high voltage ON, QX
9018395    Key, Service, yellow, QX
9012258    Worktable mount    Adapter for acmodating items with base dimensions of 100 x 150 mm or smaller on the worktable; larger items may be acmodated using multiple adjacent worktable mounts
9012189    Abdeckplatte Nadelantrieb P1xxx
950280    VX Carrier RNA (5 x 1 mg)
37551    Qproteome Mannose Glycoprotein Kit
9015326    Sensor, photo, EE-SX672A
239135    GelPilot Mid Range Ladder (100)    Ready-to-use DNA marker, 600μl (100lanes), 6 fragments: 100 – 2000 bp
239125    GelPilot Wide Range Ladder (100)    Ready-to-use DNA marker, 600μl (100lanes), 7 fragments: 200 – 4500 bp
133012    NeXtalStock Ammonium bromide (200)
133010    NeXtalStock ADA untit. (200)
133005    NeXtalStock MES untit. (200)
9011957    SamTrak Rack for twelve 16mm tubes
9011950    Modul, Plate stacker (dark), 10 plates
971510    therascreen? GIST RapidScreen Pyro Kit
36915    96-Well Magnet    Magnet for separating magnetic beads in wells of 96-well plates, 2 x 96-Well Microplates FB
950097    X-tractor Gene Consumables    The Xtractor Gene Consumables provides fully automated viral nucleic acid purification on the QIAxtractor when used with the Reagent Pack VX (#950207), and gDNA purification when used with the Reagent Pack DX (#950107). The bination of reagents and plasticware enables purification of viral nucleic acids from liquid samples, with a throughput of 8-96 samples per run.
800405    Library, Small Insert, add'tl 96 clones
800385    Sequence Comparison
9016529    Transportation box, BioRobot M48
9016525    Transportation box , BR8000/MDx
9016523    Round Carrier Set (4), 10 x 15 ml Falcon
9016517    Plate Holder Set (2)
9016300    Board, X-Main, Immucor, P3141 SI
9016203    Fitting, PCV Tubing, M6x1, 4.75mm (10)
9016148    #App. Package, M48, Genomic Research
9016117    Tip-Tray Holder, 10 μl tips    Holder for acmodating a tip tray containing 96 disposable tips of 10 μl capacity
9011914    Manual, user, BR9604, basic, US
9011874    Calibration pyramide, BR9600
9019146    Cable kit - Power, PyroQ96MD
9019139    Cable kit - Power, PyroQ96ID
9019132    Gasket - Cartridge, PyroQ96ID
9019022    Worktable cover, EZ1xl
9018999    Cover, front, bottom, EZ1xl
9018958    Master Mix Block, 1x5ml, 4x1.5ml Tubes
9018939    Adapter, LightCycler 96 Capillary Rack
9022709    Plate, Lumicheck Actuator, DML3000
9015786    Teaching Tool Samtrack
9015538    Worktable, plete
9015532    Cover, back, type B
9015526    Board, motor driver
9013861    Drawer, Complete, BR8 RD v1.0
9021146    Bottle, Liquid Waste, DCU
9021122    Valve Manifold, Lower, DCU
9021111    Skin Panel, Upper Left Side, DCU
9021106    Skin Panel, PC Mount Plain, DCU

同類產(chǎn)品推薦
在找 衣原體蛋白酶樣活性因子(CPAF)ELISA檢測試劑盒 產(chǎn)品的人還在看

提示

×

*您想獲取產(chǎn)品的資料:

以上可多選,勾選其他,可自行輸入要求

個人信息: