抗中性粒細(xì)胞核周抗體（pANCA）ELISA檢測(cè)試劑盒

抗中性粒細(xì)胞核周抗體（pANCA）ELISA檢測(cè)試劑盒齊一生物科技有限公司產(chǎn)品的新老客戶，可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù)，可檢測(cè)人源，小鼠，大鼠，豬，狗，猴，兔，羊，雞，牛，鴨子，魚，植物等各種樣本.全程提供.如需其他Elisa試劑盒說(shuō)明書及報(bào)價(jià)，可齊一生物科技有限公司.全國(guó)：歡迎選購(gòu)！

產(chǎn)品名稱：抗中性粒細(xì)胞核周抗體（pANCA）ELISA檢測(cè)試劑盒
規(guī)格：96T/48T
檢測(cè)標(biāo)本：血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測(cè)方法：ELISA
檢測(cè)類型：酶聯(lián)免疫夾心法
運(yùn)輸保存：2~8°保存6個(gè)月
齊一生物主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒全國(guó)：,種類齊全,價(jià)格實(shí)惠,購(gòu)買齊一生物產(chǎn)品的新老客戶,可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),可檢測(cè)人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚,植物等各種樣本.全程提供.如需其他Elisa試劑盒說(shuō)明書及報(bào)價(jià),可齊一生物.
特點(diǎn)
一、高效、靈敏、特異的抗體；
二、穩(wěn)定的重復(fù)性和可靠性；
三、吸附性能好,空白值低,孔底透明度高的固相載體；
四、適用血清、血漿、組織勻漿液、細(xì)胞培養(yǎng)上清液、尿液等等多種標(biāo)本類型；
五、節(jié)省實(shí)驗(yàn)經(jīng)費(fèi).
elisa試劑盒回收率是反應(yīng)待測(cè)物在樣品分析過(guò)程中的損失的程度,損失越少,回收率越高,如果作標(biāo)液1PPM,就是1毫克/升,而作出標(biāo)準(zhǔn)數(shù)據(jù)為0.99毫克/升,就是說(shuō)你的回收率是99%,這個(gè)與真實(shí)成分有密切的關(guān)系,說(shuō)明方法的準(zhǔn)確度.比如水中總無(wú)機(jī)氯含量測(cè)定,樣品水中含有無(wú)機(jī)氯20mg/L,取100mL被測(cè)水樣品,加入0.1mL濃度為10mg/mL的含無(wú)機(jī)氯標(biāo)準(zhǔn)樣品,測(cè)定時(shí)忽略體積變化,如果測(cè)定出樣品中無(wú)機(jī)氯為2.98mg/L,則認(rèn)為回收率為99%.
制備方法
包括以下步驟：
1)抗原表位的計(jì)算機(jī)篩選；
2)抗原的制備；
3)血清的采集；
4)間接ELISA方法的建立；
5)間接ELISA方法的敏感性和特異性驗(yàn)證；
6)試劑盒的穩(wěn)定性驗(yàn)證；
7)對(duì)屠宰場(chǎng)進(jìn)行臨床檢測(cè).該方法簡(jiǎn)單、快速、靈敏度高、特異性強(qiáng)、穩(wěn)定性合格、重復(fù)性好.應(yīng)用本發(fā)明制得的試劑盒能有效檢出感染豬的戊型肝炎病毒,適用于大批量發(fā)病樣本的檢測(cè),可用于豬戊肝的快速診斷,并預(yù)防、控制豬戊肝病毒的流行和阻斷戊肝病毒由豬向人傳播.
組成結(jié)構(gòu)
1、血清：操作過(guò)程中避免任何細(xì)胞刺激.使用不含熱原和內(nèi)毒素的試管.收集血液后,1000×g離心10分鐘將血紅細(xì)胞迅速小心地分離.
2、血漿：EDTA、檸檬酸鹽、肝素血漿可用于檢測(cè).1000×g離心30分鐘去除顆粒.
3、細(xì)胞上清液：1000×g離心10分鐘去除顆粒和聚合物.
4、組織勻漿：將組織加入適量生理鹽水搗碎.1000×g離心10分鐘,取上清液.
5、保存：如果樣品不立即使用,應(yīng)將其分成小部分-70℃保存,避免反復(fù)冷凍.盡可能的不要使用溶血或高血脂血.如果血清中大量顆粒,檢測(cè)前先離心或過(guò)濾.不要在37℃或更高的溫度加熱解凍.應(yīng)在室溫下解凍并確保樣品均勻地充分解凍.

9019086    PyroMark Q96 MD Software (5)    "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.

5 licenses"
9019658    O rings - VPT Fast connector, PyroVW
972012    PyroMark Q96 CpG p16 (96)    For quantification of methylation level in p16
979003    PyroMark Q96 Sample Prep Tool Cover (10)    Consumables for use with magnetic tool for isolation of single stranded DNA.
979008    PyroMark Wash Buffer (conc., 200 ml)    For washing and neutralizing the immobilized DNA.
950140    Binding Additive (DX) (4.0 g)
9019803    Upgrade Package 1, RCS
130803    NeXtal CubicPhase 1w Mo μplate (10)
9243044    Set, brackets, transparent panel, Qsym
283615    virotype PEDV/TGEV RT-PCR Rgt (96)
52906    QIAamp Viral RNA Mini Kit (250)    For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
63183    DyeEx 96 Kit (24)    24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
69582    DNeasy 96 Blood & Tissue Kit (12)    For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
70042    Oligotex mRNA Midi Kit    For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
79523    Small Spin Columns    24 RNase-free spin columns for Oligotex spin procedures
301109    PolyFect Transfection Reagent (10x100ml)
9239361    PCB-micro-fuse T 6.3A, 250V, EZ1
132632    NeXtal Stock Potassium formate (50)    10M Potassium formate - 50mL (4 x 12,5 ml tube)
9019610    Cover, Pipettor, 1 ch. (white), CAS
9019624    PCB, lge breakout, 12 core to rev 3, Cx2
9019067    PyroMark CpG SW 1.0    Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
9019087    PyroMark IdentiFire SW 1.0    Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
972052    PyroMark Q96 CpG Prader Willi (96)    For quantification of methylation level in  Prader-Willi and Angelman Syndromes
979002    PyroMark Q96 Plate Low (100)    For sample analysis of DNA template on ID.
979004    PyroMark Q96 Cartridge (3)    For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
9001513    PyroMark Q24 MDx    For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
990610    Filter-Tips, OnCor C, 200 μl (960)
9017842    Tool, gauge, 30 mm, calibration, QIAC
9020806    Rotor Timing Calibration Dye, RGQ
331455    miScript Microfluidics PreAMP Kit (96)
381566    Investigator ESSplex SE GO! Kit (200)
9022654    Transfer Carriage QIAplate, QXT
16191    QIAwell 96 Ultra Plasmid Kit (4)    For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
19046    Plasmid Buffer Set    Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps
19201    Collection Tubes (2-ml)    1000 Collection Tubes (2 ml)
9015862    EZ1 DNA PRFN Card    Pre-programmed card for BioRobot EZ1 paraffin section protocols
67163    MagAttract 96 DNA Plant Core Kit (24)    MagAttract Suspension A and buffers for 24 x 96 preps
1030483    Elution Microtubes CL, PAX, FLU
9232687    Hood f. Tip Disposal Station 2.01
9015972    Tube Holder, STS MDx, 15 mm (12)
158926    Cell Suspension Solution (125 ml)
159992    Generation DNA Purif. Soln 1 (1000 ml)
201913    dNTP Set, PCR Grade 100mM, (4x250μl)    dNTP Set is a plete set of individual, highly pure dNTPs: 100 mM each dATP, dCTP, dGTP, dTTP for 2500 x 50ul PCR reactions
12945    QIAGEN Plasmid Plus Midi Kit (100)    "100 QIAGEN Plasmid Plus Midi Columns, Extender Tubes, Reagents, Buffers, 100 QIAfilter Midi Cartridges

QIAGEN Plasmid Plus Kits enable ultrafast, large-scale plasmid purification of pure plasmid DNA. The use of a vacuum manifold allows purification of up to 12 (Mega and Giga) or up to 24 (Midi and Maxi) samples in parallel with low hands-on needs. Low elution volumes yield highly concentrated plasmid DNA for direct use without ethanol precipitation. QIAGEN Plasmid Plus Kits also feature a novel wash buffer for endotoxin reduction."
9019715    PyroMark Q24 User Manual
9019861    Rotor Locking Nut Set