現(xiàn)貨基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)ELISA檢測(cè)試劑盒齊一生物的ELISA試劑盒是您的*選擇:每個(gè)試劑盒都經(jīng)過(guò)嚴(yán)格的質(zhì)檢控制,包括:板內(nèi)、板間實(shí)驗(yàn)精度,靈敏度,回收率,穩(wěn)定性等指標(biāo)的測(cè)定。我們提供的96孔板已經(jīng)預(yù)包被好捕獲抗體,三步法檢測(cè)體系簡(jiǎn)單而直接,可根據(jù)450 nm波長(zhǎng)的讀值迅速計(jì)算定量結(jié)果。試劑盒中提供的檢測(cè)抗體能夠識(shí)別全長(zhǎng)靶蛋白的抗原決定簇以及它們的三維結(jié)構(gòu),使您的檢測(cè)結(jié)果
產(chǎn)品名稱:現(xiàn)貨基質(zhì)金屬蛋白酶抑制因子3(TIMP-3)ELISA檢測(cè)試劑盒
規(guī) 格:96T/48T
檢測(cè)標(biāo)本:血清,血漿,尿液,胸腹水,腦脊液,細(xì)胞培養(yǎng)上清,組織勻漿等
檢測(cè)方法:ELISA
檢測(cè)類(lèi)型:酶聯(lián)免疫夾心法
產(chǎn)品的用途:僅供科研究課題使用!
價(jià)格:電議,,到我司qysw@qiyibio. .齊一生物科技(上海)有限公司提供的試劑盒受到了廣大科研單位的*肯定和認(rèn)同。*保證,價(jià)格公道,傾力為國(guó)內(nèi)外科研院校實(shí)驗(yàn)室提供的產(chǎn)品。若有需要,我司將竭誠(chéng)為您服務(wù)本試劑僅供研究使用目的:本試劑盒用于測(cè)定血清,血漿及相關(guān)液體樣本中含量。
實(shí)驗(yàn)原理:本試劑盒應(yīng)用雙抗體夾心法測(cè)定標(biāo)本中該產(chǎn)品水平。用純化的本產(chǎn)品抗體包被微孔板,制成固相抗體,往包被單抗的微孔中依次加入本產(chǎn)品抗原,再與HRP標(biāo)記的本產(chǎn)品抗體結(jié)合,形成抗體-抗原-酶標(biāo)抗體復(fù)合物,經(jīng)過(guò)*洗滌后加底物TMB顯色。TMB在HRP酶的催化下轉(zhuǎn)化成藍(lán)色,并在酸的作用下轉(zhuǎn)化成zui終的黃色。顏色的深淺和樣品中的本產(chǎn)品呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(OD值),通過(guò)標(biāo)準(zhǔn)曲線計(jì)算樣品中該產(chǎn)品濃度。
試劑盒組成:
試劑盒組成 48孔配置 96孔配置 保存
說(shuō)明書(shū) 1份 1份
封板膜 2片(48) 2片(96)
密封袋 1個(gè) 1個(gè)
酶標(biāo)包被板 1×48 1×96 2-8℃保存
標(biāo)準(zhǔn)品:1800ng/L 0.5ml×1瓶 0.5ml×1瓶 2-8℃保存
標(biāo)準(zhǔn)品稀釋液 1.5ml×1瓶 1.5ml×1瓶 2-8℃保存
酶標(biāo)試劑 3 ml×1瓶 6 ml×1瓶 2-8℃保存
樣品稀釋液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
顯色劑A液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
顯色劑B液 3 ml×1瓶 6 ml×1瓶 2-8℃保存
終止液 3ml×1瓶 6ml×1瓶 2-8℃保存
濃縮洗滌液 (20ml×20倍)×1瓶 (20ml×30倍)×1瓶 2-8℃保存
標(biāo)本要求:
1.標(biāo)本采集后盡早進(jìn)行提取,提取按相關(guān)文獻(xiàn)進(jìn)行,提取后應(yīng)盡快進(jìn)行實(shí)驗(yàn)。若不能馬上進(jìn)行試驗(yàn),可將標(biāo)本放于-20℃保存,但應(yīng)避免反復(fù)凍融
2.不能檢測(cè)含NaN3的樣品,因NaN3抑制辣根過(guò)氧化物酶的(HRP)活性。
注意事項(xiàng):
1. 試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用,酶標(biāo)包被板開(kāi)封后如未用完,板條應(yīng)裝入密封袋中保存。
2. 濃洗滌液可能會(huì)有結(jié)晶析出,稀釋時(shí)可在水浴中加溫助溶,洗滌時(shí)不影響結(jié)果。
3. 各步加樣均應(yīng)使用加樣器,并經(jīng)常校對(duì)其準(zhǔn)確性,以避免試驗(yàn)誤差。一次加樣時(shí)間控制在5分鐘內(nèi),如標(biāo)本數(shù)量多,推薦使用排槍加樣。
4. 請(qǐng)每次測(cè)定的同時(shí)做標(biāo)準(zhǔn)曲線,做復(fù)孔。如標(biāo)本中待測(cè)物質(zhì)含量過(guò)高(樣本OD值大于標(biāo)準(zhǔn)品孔*孔的OD值),請(qǐng)先用樣品稀釋液稀釋一定倍數(shù)(n倍)后再測(cè)定,計(jì)算時(shí)請(qǐng)zui后乘以總稀釋倍數(shù)(×n×5)。
5. 封板膜只限一次性使用,以避免交叉污染。
6. 底物請(qǐng)避光保存。
7. 嚴(yán)格按照說(shuō)明書(shū)的操作進(jìn)行,試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).
8. 所有樣品,洗滌液和各種廢棄物都應(yīng)按傳染物處理。
9. 本試劑不同批號(hào)組分不得混用。
10. 如與英文說(shuō)明書(shū)有異,以英文說(shuō)明書(shū)為準(zhǔn)。
計(jì)算:
以標(biāo)準(zhǔn)物的濃度為橫坐標(biāo),OD值為縱坐標(biāo),
在坐標(biāo)紙上繪出標(biāo)準(zhǔn)曲線,根據(jù)樣品的OD
值由標(biāo)準(zhǔn)曲線查出相應(yīng)的濃度;再乘以稀釋
倍數(shù);或用標(biāo)準(zhǔn)物的濃度與OD值計(jì)算出標(biāo)
準(zhǔn)曲線的直線回歸方程式,將樣品的OD值
代入方程式,計(jì)算出樣品濃度,再乘以稀釋
倍數(shù),即為樣品的實(shí)際濃度。 試劑盒性能:
1.樣品線性回歸與預(yù)期濃度相關(guān)系數(shù)R值為0.95以上。
2.批內(nèi)與批見(jiàn)應(yīng)分別小于9%和11%
保存條件及有效期:
1.試劑盒保存;2-8℃。
2.有效期:6個(gè)月 齊一生物科技有限公司主要經(jīng)營(yíng)進(jìn)口原裝Elisa試劑盒,種類(lèi)齊全,價(jià)格實(shí)惠,購(gòu)買(mǎi)我公司產(chǎn)品的新老客戶,可享免費(fèi)代測(cè)實(shí)驗(yàn)服務(wù),可檢測(cè)人源,小鼠,大鼠,豬,狗,猴,兔,羊,雞,牛,鴨子,魚(yú),植物等各種樣本。全程提供。如有什么問(wèn)題請(qǐng)給我們或者留言,告訴我您的需要,并留下您寶貴的意見(jiàn)和建議。
Accessory 19577 48-Well Blocks (24) 48-well blocks with 5 ml wells, 24 per case
Instrument Part 9017734 Plasticware kit, IQ/OQ, EZ1
Instrument Part 9019885 Cover, main, label Biocontrol, RG3
Instrument Part 9019907 Switch - Vacuum VPT, PyroQ24VW
Instrument Part 9019012 Motor Y, EZ1xl
Instrument Part 9019025 Worktable, side wall, left, EZ1xl
Instrument Part 9019729 Board, motor control, Z, V2, QX
9019745 EZ1 Adv. XL DNA Dried Blood Card
Consumable 870501 ALK RGQ RT-PCR Kit (24)
Accessory 36912 d12-Tube Magnet Magnet for separating magnetic beads in 12 x 1.5 ml or 2 ml tubes
Consumable 51185 QIAamp DNA Blood Midi Kit (100) For 100 DNA midipreps: 100 QIAamp Midi Spin Columns, QIAGEN Protease, Buffers, Collection Tubes (15 ml)
Consumable 68163 DNeasy Plant Maxi Kit (24) 24 DNeasy Maxi Spin Columns, 24 QIAshredder Maxi Spin Columns, RNase A, Buffers, Collection Tubes (50 ml)
Consumable 79216 Buffer RLT 220 ml RNeasy Lysis Buffer
Accessory 120007 Collection Microtubes and Caps Nonsterile polypropylene tubes (1.2 ml), 2304 in packs of 96, and nonsterile polypropylene caps for Collection Microtubes
Consumable 201207 Taq DNA Polymerase (5000) "20 x 250 units Taq DNA Polymerase, 10x PCR Buffer,* 5x Q-Solution, 25 mM MgCl2
NEW! Now with 10x CoralLoad PCR Buffer
Taq DNA Polymerase is now supplied with CoralLoad PCR Buffer, which has all of the advantages of QIAGEN PCR Buffer but can also be used to directly load the PCR reaction onto an agarose gel without the need to add a gel loading buffer. CoralLoad PCR Buffer provides the same high PCR specificity and minimal reaction optimization as conventional QIAGEN PCR Buffer. Additionally, it contains two marker dyes: an orange dye and a red dye, that facilitate estimation of DNA migration distance and optimization of agarose gel run time."
Consumable 132559 NeXtal Stock Magnesium acetate (50) 1M Magnesium acetate tetrahydrate - 50mL (4 x 12,5 ml tube)
Instrument Part 9019585 Slideout electrical tray, QGY
Instrument Part 9019599 Housing, filter seal, CX1
Instrument Part 9019602 Home flag, Y-axis, Cx8
Instrument Part 9019649 Fuse kit (6.3Ax10, 5Ax5), PyroQ96
Consumable 970012 PyroMark Q24 CpG p16 (4 x 24) For quantification of methylation level in region +148 to +174 in exon 1 of the gene.
Instrument Part 972824 PyroMark Gold Q96 CDT Reagents (6 x 96) For performing pyrosequencing reaction on the Q96 MD in bination with the capillary dispensing tips (CDT)
Instrument Part 9019668 PCB - Valve, PyroQ24
Consumable 950911 Capture Plate, DX (5)
Instrument Part 981313 Rotor-Disc 100 (300)
1070498 Buffer TCL, 2x (8.5 ml),G
Consumable 206624 Type-it CNV SYBR Green PCR Core Kit(400)
Consumable 19055 Buffer QC 1000 ml Wash Buffer
Consumable 13362 Blood & Cell Culture DNA Maxi Kit 10 QIAGEN Genomic-tip 500/G, QIAGEN Protease, Buffers
Consumable 19064 Buffer N3 500 ml Neutralization Buffer
Consumable 19081 Buffer AW1 242 ml Wash Buffer (1) Concentrate for 1000 spin, 250 midi, or 100 maxi preps
Accessory 19530 Vacuum Regulator For use with QIAvac manifolds
Consumable 28304 QIAquick Nucleotide Removal Kit (50) 50 QIAquick Spin Columns, Buffers, Collection Tubes (2 ml)
Instrument Part 9012730 Cable, flat band, X, 2300 mm
Instrument Part 9013173 Pump, probe flush, BR8000
MDx 4510033 artus Enterovirus LC RT-PCR Kit (24) PRE
Consumable 130718 NeXtal Tubes PACT Suite
Consumable 19088 Reagent DX
Consumable 218411 Human miScript Primer Assay Set VXX.X
Instrument Part 9019899 Cable - Ground, PyroQ96ID
Instrument Part 9019003 Hinge, for door, EZ1xl
Instrument Part 9019004 Grid, UV lamp, EZ1xl
Instrument Part 9019021 Damper, door, EZ1xl
Instrument Part 9019759 Power supply, main, 24V, V2, QX
Consumable 950154 Wash Reagent (DXW) (650 ml)
Instrument Part 9019537 Plate Stacker A/B RD, RCS
Instrument Part 9021031 Frame, transfer module, Qsym
Consumable 337042 qBiomarker Assay PreAMP Mix
Consumable 51161 QIAamp 96 DNA Blood Kit (4) For 4 x 96 DNA preps: 4 QIAamp 96 Plates, QIAGEN Protease, Reagents, Buffers, Lysis Blocks, Tape Pads, Collection Vessels
Consumable 75162 RNeasy Maxi Kit (12) 12 RNeasy Maxi Spin Columns, Collection Tubes (50 ml), RNase-free Reagents and Buffers
Consumable 120016 QIAquick 96 Plates (24) Individual QIAquick 96 plates and handbook. No buffers or reagents.
Consumable 203443 HotStarTaq Master Mix Kit (250) "3 x 0.85 ml HotStarTaq Master Mix* containing 250 units HotStarTaq DNA Polymerase total, 2 x 1.7 ml distilled water
- premixed solution for high PCR specificity
- HotStarTaq Master Mix, HotStarTaq DNA Polymerase, several kit sizes from 250 u up to 2500 u available"
Consumable 301108 PolyFect Transfection Reagent (100 ml) For 2500-6500 transfections in 60 mm dishes or 5000-10,000 transfections in 6-well plates.
Consumable 231222 QIAGEN PCR Cloning plus Kit (10) For 10 reactions: 2x Ligation Master Mix (50 ul), pDrive Cloning Vector (0.5 ug), distilled water (1.7 ml), QIAGEN EZ Competent Cells (10 tubes, 50 ul each), SOC medium (2 x 1.9 ml)
Instrument Part 9015638 AC fuse, 115 V, 250 8A, M48
Consumable 132568 NeXtal Stock Methoxy-PEG 5000 (50) 30% w/v Methoxypolyethylene glycol 5000 - 50mL (4 x 12,5 ml tube)
Instrument Part 9019577 Opto switch, Z-axis, 1 ch. , Cx2
Instrument Part 9019594 Belt, Z-axis gearbox (black), 8 ch., Cx8
Consumable 9019068 PyroMark CpG SW 1.0 (5) "Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
Five additional software licenses for PyroMark CpG SW 1.0"
Consumable 9019086 PyroMark Q96 MD Software (5) "For use with PyroMark Q96 MD instrument.Software for setting up a run and analyzing mutations.
5 licenses"
Instrument Part 9019658 O rings - VPT Fast connector, PyroVW
Consumable 972012 PyroMark Q96 CpG p16 (96) For quantification of methylation level in p16
Consumable 979003 PyroMark Q96 Sample Prep Tool Cover (10) Consumables for use with magnetic tool for isolation of single stranded DNA.
Consumable 979008 PyroMark Wash Buffer (conc., 200 ml) For washing and neutralizing the immobilized DNA.
950140 Binding Additive (DX) (4.0 g)
Instrument Part 9019803 Upgrade Package 1, RCS
Consumable 130803 NeXtal CubicPhase 1w Mo μplate (10)
Instrument Part 9243044 Set, brackets, transparent panel, Qsym
Consumable 283615 virotype PEDV/TGEV RT-PCR Rgt (96)
Consumable 52906 QIAamp Viral RNA Mini Kit (250) For 250 RNA preps: 250 QIAamp Mini Spin Columns, Carrier RNA, Collection Tubes (2 ml), RNase-free Buffers
Consumable 63183 DyeEx 96 Kit (24) 24 DyeEx 96 Plates; 4 Collection Plates, 48-Well
Consumable 69582 DNeasy 96 Blood & Tissue Kit (12) For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, QIAGEN Proteinase K, Buffers, Square-Well Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Caps, 96-well Plate Registers
Consumable 70042 Oligotex mRNA Midi Kit For 12 mRNA midipreps: 700 ul Oligotex Suspension, Small Spin Columns, Collection Tubes (1.5 ml), RNase-free Reagents and Buffers
Accessory 79523 Small Spin Columns 24 RNase-free spin columns for Oligotex spin procedures
Consumable 301109 PolyFect Transfection Reagent (10x100ml)
Instrument Part 9239361 PCB-micro-fuse T 6.3A, 250V, EZ1
Consumable 132632 NeXtal Stock Potassium formate (50) 10M Potassium formate - 50mL (4 x 12,5 ml tube)
Instrument Part 9019610 Cover, Pipettor, 1 ch. (white), CAS
Instrument Part 9019624 PCB, lge breakout, 12 core to rev 3, Cx2
Consumable 9019067 PyroMark CpG SW 1.0 Software for PyroMark Q96 ID and PyroMark Q96 MD for analysis and quantification of CpG methylation in epigenetic studies.
Consumable 9019087 PyroMark IdentiFire SW 1.0 Software for sequence-based species identification by paring and matching Pyrosequencing data to a local database.
Consumable 972052 PyroMark Q96 CpG Prader Willi (96) For quantification of methylation level in Prader-Willi and Angelman Syndromes
Consumable 979002 PyroMark Q96 Plate Low (100) For sample analysis of DNA template on ID.
Consumable 979004 PyroMark Q96 Cartridge (3) For delivery of nucleotides and reagents for use in PyroMark Q96 ID.
Consumable 9001513 PyroMark Q24 MDx For methylation and mutation analysis using Pyrosequencing technology in 24 well CE-marked.
Consumable 990610 Filter-Tips, OnCor C, 200 μl (960)
Instrument Part 9017842 Tool, gauge, 30 mm, calibration, QIAC
Instrument Part 9020806 Rotor Timing Calibration Dye, RGQ
Consumable 331455 miScript Microfluidics PreAMP Kit (96)
381566 Investigator ESSplex SE GO! Kit (200)
Instrument Part 9022654 Transfer Carriage QIAplate, QXT
Consumable 16191 QIAwell 96 Ultra Plasmid Kit (4) For 4 x 96 ultrapure plasmid minipreps, 4 each: QIAfilter 96, QIAwell 96, and QIAprep 96 Plates; Reagents, Buffers, Collection Microtubes (1.2 ml), Caps
Consumable 19046 Plasmid Buffer Set Buffers P1, P2, P3, QBT, QC, QF, RNase A; for 100 plasmid mini-, 25 midi-, or 10 maxipreps